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HelpTest Code LAB12313761 Chlamydia/Neisseria gonorrhoeae (GC) DNA Probe,Urine
Useful For
Testing at RRMC is for 14 years and OLDER. 13 years and under are collected in GenProbe Aptima II media and sent to Lab Corp; Order LAB1234555 and check with Central Processing to obtain special collection media.
Use: Qualitative detection of plasmid DNA for C. trachomatis/GC in urogenital (female) samples or urine (male or female) samples.
PCR method should not be used as a “test for cure.”
Urine
Given that collection of larger volumes of urine may reduce test sensitivity, urine collected in volumes greater than 50 mls may cause false negative results.
Note: Grossly bloody samples are unacceptable and may yield false positive results. Grossly mucoid samples are unacceptable and may yield false negative results. Avoid sample contamination, the presence of spermicide, lubricants or feminine hygiene powders.
Performing Laboratory
Asante Rogue Regional Medical Center (ARRMC)
Performing Department
Microbiology
Specimen Required
Male or female non-clean catch ( “dirty”) urine specimens collected in a clean, polypropylene container without preservatives or transported in Cobas PCR media (instructions follow)
For males, only urine is acceptable. Not an approved method for male urogenital samples.
Acceptable: Sterile Urine Cup Sterile collection cup
Chlamydia/GC URINE sample- Male/Female: GC-Chlamydia Urine (male or female) collection
Dirty catch urine, preferred collection after 1 hour of not-voiding, volume between 15-50 mls, (dirty catch, first part of the stream, not mid-stream) into a clean, polypropylene container without preservatives. (You may want to mark the outside of the cup to show the desired volume.) Too much urine volume causes a dilution effect and can yield negative results. Caution the patient not to begin urinating until the collection cup is in position.
Urine can be submitted directly to Asante RRMC lab. Aliquot urine into Cobas PCR® media to only fill between the lines (do not overfill) within 24 hours of collection. Office may submit urine already aliquoted in Cobas PCR® media. DO NOT OVERFILL the Cobas PCR® media vial; fill ONLY in between the lines. NOTE: Given that collection of larger volumes of urine may reduce test sensitivity, emphasis should be placed on the FIRST voided part of the stream.
Date and label the tube with appropriate patient identification information.
Also include specimen source information on transport tube and on test requisition.
Specimen Minimum Volume
10 mL non-clean catch urine
Specimen Stability Information
Transport to the laboratory as soon as possible.
Specimens collected and transported with the Cobas® PCR Sample Kit may be stored at 2°- 30°C (refrigerated or room temperature) for up to 12 months once the specimens have been stabilized in Cobas® PCR® Media.
If a specimen cannot be stabilized immediately than refrigerate the urine and transport it to the laboratory as soon as possible. Stability of urine, not stabilized in Cobas PCR® media is 2°- 30°C for up to 24 hours.
Reject Due To
Clean-catch urine; this methodology has not been evaluated for this specimen type
Day(s) Performed
Monday - Friday
All positive samples results are phoned to the physician.
Positive results also require Public Health reporting and follow-up.
Method Name
Real-time PCR (Polymerase Chain Reaction)
CPT Code Information
- 87491: Infectious agent detection by nucleic acid (DNA or RNA); Chlamydia trachomatis, amplified probe technique
- 87591 Infectious agent detection by nucleic acid (DNA or RNA); Neisseria gonorrhoeae, amplified probe technique
Billing Code
- 3060024
- 3060087