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General Information

Mayo Clinic Laboratories

Test Code TOXB Toxoplasma gondii, Molecular Detection, PCR, Blood

Useful For

Supporting the diagnosis of active toxoplasmosis, particularly in immunocompromised individuals

Reporting Name

Toxoplasma gondii PCR, B

Specimen Type

Whole Blood EDTA


Specimen Required


Container/Tube: Lavender top (EDTA)

Specimen Volume: 1 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.


Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood EDTA Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Reference Values

Negative

Supportive Data

Accuracy/Diagnostic Sensitivity and Specificity:

Accuracy was determined using a combination of spiking and clinical specimens for each source accepted for testing. Accuracy ranges from 97% to 100%.

 

Analytical Sensitivity/Limit of Detection:

The limit of detection for this assay is less than 5000 copies/mL

 

Analytical Specificity:

No PCR signal was obtained from extracts of 20 bacterial, parasitic, and viral isolates from similar organisms and from organisms commonly found in the specimen types tested.

 

Precision:

Intra-assay precision and interassay precision are 100%.

 

Reference Range:

The reference range is "Negative" for this assay.

 

Reportable Range:

This is a qualitative assay and results are reported as "Negative" or "Positive."

Day(s) Performed

Monday through Saturday

Report Available

Same day/1 to 4 days

Specimen Retention Time

1 week

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TOXB Toxoplasma gondii PCR, B 16279-2

 

Result ID Test Result Name Result LOINC Value
62977 Toxoplasma gondii PCR, B 16279-2

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.