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Test Code TOXB Toxoplasma gondii, Molecular Detection, PCR, Blood

Useful For

Supporting the diagnosis of active toxoplasmosis, particularly in immunocompromised individuals

Reporting Name

Toxoplasma gondii PCR, B

Specimen Type

Whole Blood EDTA


Specimen Required


Container/Tube: Lavender top (EDTA)

Specimen Volume: 1 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.


Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Reference Values

Negative

Supportive Data

Accuracy/Diagnostic Sensitivity and Specificity:

Accuracy was determined using a combination of spiking and clinical specimens for each source accepted for testing. Accuracy ranges from 97% to 100%.

 

Analytical Sensitivity/Limit of Detection:

The limit of detection for this assay is less than 5000 copies/mL

 

Analytical Specificity:

No PCR signal was obtained from extracts of 20 bacterial, parasitic, and viral isolates from similar organisms and from organisms commonly found in the specimen types tested.

 

Precision:

Intra-assay precision and interassay precision are 100%.

 

Reference Range:

The reference range is "Negative" for this assay.

 

Reportable Range:

This is a qualitative assay and results are reported as "Negative" or "Positive."

Day(s) Performed

Monday through Saturday

Report Available

Same day/1 to 4 days

Specimen Retention Time

1 week

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TOXB Toxoplasma gondii PCR, B 16279-2

 

Result ID Test Result Name Result LOINC Value
62977 Toxoplasma gondii PCR, B 16279-2

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.