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HelpTest Code PNTO Streptococcus pneumoniae IgG Antibodies, Total, Serum
Ordering Guidance
This test is the preferred test for patients previously tested for Streptococcus pneumoniae antibodies (as part of follow up testing or part of pre/post vaccine assessment).
The preferred test for patients being evaluated for possible immunodeficiency or for assessment of pneumococcal vaccination response (initial evaluation) is PNTOR / Streptococcus pneumoniae IgG Antibodies, Total, with Reflex, Serum.
The preferred test for patients previously tested for S pneumoniae serotypes (as part of follow up testing or part of pre/post vaccine assessment) is PN23M / Streptococcus pneumoniae IgG Antibodies, 23 Serotypes, Serum.
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Useful For
Assessing the IgG antibody response to active immunization with nonconjugated 23-valent pneumococcal vaccines
Assessing the IgG antibody response to active immunization with conjugated 13-valent, 15-valent and 20-valent pneumococcal vaccines
Determining the ability of an individual to produce an antibody response to polysaccharide antigens, as part of an evaluation for humoral or combined immunodeficiencies
Reporting Name
S. pneumoniae IgG Ab, Total, SSpecimen Type
SerumSpecimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 21 days | |
| Frozen | 21 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
| Heat-inactivated specimen | Reject |
Reference Values
≥9.7 mcg/mL
Day(s) Performed
Tuesday, Thursday
Report Available
2 to 8 daysSpecimen Retention Time
12 weeksPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86317
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| PNTO | S. pneumoniae IgG Ab, Total, S | 43236-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| PNTO | S. pneumoniae IgG Ab, Total, S | 43236-9 |
Supportive Data
The utility of measuring the total IgG response to Streptococcus pneumoniae vaccination was assess in a study published by Parker et al. In a cohort of healthy individuals (n=77), a median 9-fold increase in IgG reactivity was observed 4 to 6 weeks after vaccination, with median IgG concentrations of 41 mcg/mL and 375 mcg/mL in pre- and post-vaccination samples, respectively. Based upon data in the healthy control group, a concentration of 77 mcg/mL was established as the threshold for defining a normal vaccination response. Using this cut-off, in a cohort of individuals with humoral primary immunodeficiencies, 62.7% (64/102) had results below this cut-off following vaccination, compared to 3.9% (3/77) in the healthy control group (10). While measurement of antibodies against Streptococcus pneumoniae is not diagnostic for any specific disease, it can be used to understand the nature and magnitude of immunoglobulin deficiencies in patients with suspected humoral immune disorders.