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Test Code PLPMA Prolactin, Pituitary Macroadenoma, Serum

Useful For

Quantifying prolactin in serum specimens where the high-dose hook effect is suspected (eg, presence of pituitary tumor with symptoms of prolactinoma, and lower than expected serum prolactin concentration)

Testing Algorithm

A pituitary adenoma should be identified by imaging studies prior to ordering this test.

Reporting Name

Prolactin, Pituitary Macroadenoma

Specimen Type

Serum


Ordering Guidance


For initial patient assessment; order PRL / Prolactin, Serum as the screening test.



Necessary Information


Patient's age and sex are required.



Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 2 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Specimen Minimum Volume

1.75 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 7 days
  Frozen  90 days
  Ambient  24 hours

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Gross icterus OK

Reference Values

Males

<18 years: not established

≥18 years: 4.0-15.2 ng/mL

 

Females:

<18 years: not established

≥18 years: 4.8-23.3 ng/mL 

Method Description

The Roche Cobas e immunoassay Prolactin II method employs 2 monoclonal antibodies specifically directed against prolactin. A biotinylated monoclonal antibody and a second monoclonal antibody labeled with a ruthenium complex react with prolactin in the sample to form a sandwich complex. After the addition of streptavidin-coated microparticles, the complex becomes bound to the solid phase via interaction of biotin and streptavidin. Application of a voltage to the electrode then induces chemiluminescent emission, which is measured.(Package insert: Roche E170/Cobas e601/e602 Prolactin II)

 

Dilution of serum sample is performed (10-, 100-, and 400-fold dilution) using manufacturer-recommended diluent and recovery of prolactin is compared to the prolactin concentration in neat sample. Acceptable recovery is defined as 100 + or - 20% recovery after dilution of serum.

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 to 2 days

Specimen Retention Time

6 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

84146

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PLPMA Prolactin, Pituitary Macroadenoma 20568-2

 

Result ID Test Result Name Result LOINC Value
PRLR Prolactin, S 20568-2
CMT89 Comment 48767-8

Forms

If not ordering electronically, complete, print, and send an Oncology Test Request (T729) with the specimen.