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HelpTest Code PLAFL Platelet Surface Glycoprotein, Flow Cytometry, Blood
Useful For
Identification of markedly decreased CD41 (GPIIb) and CD61 (GPIIIa) expression levels, which are diagnostic for Glanzmann thrombasthenia
Identification of markedly decreased CD42a (GPIX) and CD42b (GPIb-alpha) expression levels, which are diagnostic for Bernard-Soulier syndrome
Identification of decreased GPVI expression, which suggests collagen receptor deficiency
Identification of decreased CD49b (GPIa), which suggests collagen receptor deficiency
Special Instructions
Reporting Name
Platelet Glycoprotein Flow, BSpecimen Type
Whole Blood ACDOrdering Guidance
This test identifies platelet surface glycoprotein or receptor deficiencies. If platelet structural abnormality testing is needed, consider PTEM / Platelet Transmission Electron Microscopic Study, Whole Blood.
Shipping Instructions
Specimen must be shipped ambient and arrive within 4 days of collection.
Ship specimen overnight in an Ambient Shipping Box-Critical Specimens Only (T668) following the instructions in the mailer.
Necessary Information
Platelet Esoteric Testing Patient Information is required. Testing may proceed without this information, however, the information aids in providing a more thorough interpretation. Ordering providers are strongly encouraged to fill out the form and send with the specimen.
Specimen Required
Supplies: Ambient Shipping Box-Critical Specimens Only (T668)
Collection Container/Tube: ACD solution A or B
Specimen Volume: 6 mL
Pediatric Volume: 1 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Specimen Minimum Volume
Adult: 1 mL; Pediatric: 200 mcL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood ACD | Ambient | 4 days |
Reject Due To
| Gross hemolysis | Reject |
| Fully clotted | Reject |
| Gross lipemia | OK |
Reference Values
GPIIb CD41: ≥70.0% (Normal Range-Median)
GPIIIa CD61: ≥70.0% (Normal Range-Median)
GPIX CD42a: ≥70.0% (Normal Range-Median)
GPIb-alpha CD42b: ≥70.0% (Normal Range-Median)
GPIa CD49b: ≥60.0% (Normal Range-Median)
Supportive Data
The platelet glycoprotein flow cytometry method was established in the Mayo Clinic Special Coagulation Laboratory in 2009. Between 2009 to 2014, a total of 155 clinical patients at Mayo Clinic were tested. The flow cytometry results were compared with the final impressions of platelet light transmission aggregation testing. There were 7 samples that had flow cytometric features of Glanzmann thrombasthenia, 2 samples that had flow cytometric features of Bernard-Soulier syndrome, and 3 samples that had flow cytometric features of May-Hegglin anomaly. All flow cytometric results were concordant with platelet light transmission aggregation results and other clinical findings.
Day(s) Performed
Monday through Saturday
Report Available
1 to 2 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
88184-Flow cytometry; first cell surface, cytoplasmic or nuclear marker
88185-Flow cytometry; additional cell surface, cytoplasmic or nuclear marker (each) X5
88187-Flow cytometry interpretation, 2 to 8 markers
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| PLAFL | Platelet Glycoprotein Flow, B | 93320-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| CK111 | GPIIb CD41 | 93319-2 |
| CK112 | GPIIIa CD61 | 93318-4 |
| CK113 | GPIX CD42a | 93317-6 |
| CK114 | GPIb-alpha CD42b | 93316-8 |
| CK115 | GPIa CD49b | 93315-0 |
| CK116 | GPVI | 93314-3 |
| CK117 | Final Diagnosis | 93313-5 |
Forms
1. Platelet Esoteric Testing Patient Information is required.
2. If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.