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HelpTest Code PARVO Parvovirus B19, Molecular Detection, PCR, Varies
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
Parvovirus B19 PCRSpecimen Type
VariesNecessary Information
Specimen source is required.
Specimen Required
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Amniotic fluid
Container/Tube: Amniotic fluid container
Specimen Volume: 0.5 mL
Collection Instructions:
1. Do not centrifuge.
2. Label specimen as amniotic fluid.
Specimen Type: Spinal fluid
Container/Tube: Sterile vial
Specimen Volume: 0.5 mL
Collection Instructions:
1. Do not centrifuge.
2. Label specimen as spinal fluid.
Specimen Type: Synovial fluid
Container/Tube: Sterile vial or lavender top (EDTA)
Specimen Volume: 0.5 mL
Collection Instructions: Label specimen as synovial fluid.
Alternate:
Specimen Type: Bone marrow
Container/Tube: Sterile container or lavender top (EDTA)
Specimen Volume: 0.5 mL
Collection Instructions: Label specimen as bone marrow.
Reject Due To
| All specimens will be evaluated at Mayo Clinic Laboratories for test suitability. |
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Refrigerated (preferred) | 7 days | |
| Frozen | 7 days |
Specimen Minimum Volume
Amniotic Fluid, Bone Marrow, and Spinal Fluid 0.3 mL; Synovial fluid 0.5 mL
Day(s) Performed
Monday through Friday
Specimen Retention Time
1 weekReport Available
Same day/1 to 3 daysReference Values
Negative
Useful For
Diagnosing parvovirus B19 infection
CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| PARVO | Parvovirus B19 PCR | 9571-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| SRC73 | Source | 31208-2 |
| 83151 | Parvovirus B19 By Rapid PCR | 9571-1 |
Supportive Data
The following data supports the use of this assay for clinical testing.
Accuracy/Diagnostic Sensitivity and Specificity:
Results from this real-time polymerase chain reaction (PCR) assay on the LightCycler (LC PCR) were compared to a Centers for Disease Control and Prevention (CDC) PCR-based assay on tissue biopsy specimens of temporal artery. Using the CDC PCR as the gold standard, the diagnostic sensitivity and specificity for detection of parvovirus B19 was 97%.
Supplemental Data:
To supplement the above data, 30 negative cerebrospinal fluid, body fluids, and tissues, and 45 negative blood specimens were spiked with parvovirus B19-positive control plasmid at the limit of detection (LOD)(10-20 targets/microliter). The 30 spiked specimens (45 bloods) were run in a blinded manner along with 30 negative (non-spiked) specimens (45 bloods). Results showed 97% to 100% of the spiked specimens were positive and 100% of the non-spiked specimens were negative.
Analytical Sensitivity/LOD:
The LOD of this assay is 10 to 20 targets/microliter in sample matrix.
Analytical Specificity:
No PCR signal was obtained with extracts of 11 viral and bacterial isolates that may cause symptoms similar to infection with parvovirus, including herpes simplex virus, varicella-zoster virus, cytomegalovirus, and human herpesvirus-6, -7, and -8.
Precision:
Interassay precision was 100% and intra-assay precision was 97%.
Reference Range:
Although the reference range is typically "negative" for this assay, it may detect viremia in asymptomatic individuals or remnant viral nucleic acid. However, this assay is only to be used for patients with a clinical history and symptoms consistent with parvovirus B19 infection and must be interpreted in context of clinical picture. This test is not to be used to screen asymptomatic patients.
Reportable Range:
This is a qualitative assay, and results are reported as either negative or positive for targeted parvovirus B19.
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.