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HelpTest Code NGAML MayoComplete Acute Myeloid Leukemia, 11-Gene Panel, Varies
Useful For
Evaluation of acute myeloid leukemia (AML) using a focused 11-gene panel at the time of diagnosis or possibly at relapsed/refractory disease to help guide classification and possible therapeutic approaches
Testing Algorithm
For a list of genes and exons targeted by this test see Targeted Genes Interrogated by Acute Myeloid Leukemia, 11-Gene Panel
Special Instructions
Reporting Name
AML, 11 Gene, NGS, VSpecimen Type
VariesOrdering Guidance
This gene panel is a subset of the NGSHM / Myeloid Neoplasms, Comprehensive OncoHeme Next-Generation Sequencing test and focuses more specifically on the gene mutations that are most prevalent and clinically significant in acute myeloid leukemias (AML). If a wider gene mutation analysis is desired or the indication for testing is for a myeloid malignancy other than AML, consider NGSHM.
Shipping Instructions
Bone marrow and peripheral blood specimens must arrive within 14 days of collection.
Necessary Information
The following information is required:
1. Clinical diagnosis
2. Pertinent clinical history, including disease phase (diagnostic, remission, relapse/refractory) and therapy status (especially if patient has received a hematopoietic stem cell transplant).
3. Clinical or morphologic suspicion
4. Date of collection
5. Specimen source
Specimen Required
Submit only 1 of the following specimens:
Preferred Specimen Type: Bone marrow aspirate
Container/Tube:
Preferred: Lavender top (EDTA) or yellow top (ACD)
Acceptable: Green top (sodium heparin)
Specimen Volume: 2 mL
Collection Instructions:
1. Invert several times to mix bone marrow.
2. Send bone marrow specimen in original tube. Do not aliquot.
3. Label specimen as bone marrow.
Specimen Stability: Ambient (preferred)/Refrigerate
Specimen Type: Peripheral blood
Container/Tube:
Preferred: Lavender top (EDTA) or yellow top (ACD)
Acceptable: Green top (sodium heparin)
Specimen Volume: 3 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood specimen in original tube. Do not aliquot.
3. Label specimen as blood.
Specimen Stability: Ambient (preferred)/Refrigerate
Specimen Type: Extracted DNA from blood or bone marrow
Container/Tube: 1.5 to 2 mL tube with indication of volume and concentration of the DNA
Specimen Volume: Entire specimen
Collection Instructions: Label specimen as extracted DNA and source of specimen.
Specimen Stability: Frozen (preferred)/Refrigerated/Ambient
Specimen Minimum Volume
Blood, Bone marrow: 1 mL
Extracted DNA: 100 mcL at 20 ng/mcL concentration
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Varies | 14 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Bone marrow biopsies Slides Paraffin shavings or frozen tissues Paraffin-embedded tissues Paraffin-embedded bone marrow aspirates Moderately to severely clotted |
Reject |
Reference Values
An interpretive report will be provided
Day(s) Performed
Monday through Friday
Report Available
16 to 21 daysSpecimen Retention Time
Peripheral blood, bone marrow: 2 weeks; DNA 3 monthsPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
81450
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| NGAML | AML, 11 Gene, NGS, V | 105343-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| MP038 | Specimen Type | 31208-2 |
| 43554 | NGAML Result | No LOINC Needed |
| 43488 | Pathogenic Mutations Detected | 82939-0 |
| 43487 | Interpretation | 69047-9 |
| 43489 | Clinical Trials | 82786-5 |
| 43490 | Variants of Unknown Significance | 93367-1 |
| 43491 | Additional Notes | 48767-8 |
| 43492 | Method Summary | 85069-3 |
| 43493 | Disclaimer | 62364-5 |
| 43494 | AML Panel Gene List | 36908-2 |
| 43495 | Reviewed By | 18771-6 |
Forms
1. Hematopathology Patient Information (T676)
2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.
Genetics Test Information
This test includes next-generation sequencing to evaluate for the following 11 genes: CEBPA, DNMT3A, FLT3, IDH1, IDH2, KIT, KRAS, NPM1, NRAS, RUNX1, and TP53.