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HelpTest Code MUMPU Mumps Virus, Molecular Detection, PCR, Urine
Ordering Guidance
If serology has been performed and IgM-class antibodies against mumps are detected (MMPGM / Mumps Virus Antibody, IgM and IgG, Serum), this test should be ordered to confirm mumps infection.
Shipping Instructions
Specimens must be shipped at refrigerated temperature. Specimens received frozen will be rejected.
Specimen Required
Supplies: Urine Tubes, 10 mL (T068)
Container/Tube: Plastic, 10-mL urine tube
Specimen Volume: 1 mL
Collection Instructions:
1. Collect a random urine specimen.
2. No preservative.
Reporting Name
Mumps Virus PCR, UrineSpecimen Type
UrineSpecimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Urine | Refrigerated | 7 days |
Reject Due To
| Samples that have been heat-inactivated Urine containing preservatives |
Reject |
Reference Values
Negative
Supportive Data
The following validation data support the use of this assay for clinical testing.
Accuracy:
Accuracy studies were performed by testing negative and positive (near the limit of detection) urine and buccal swab samples. Both urine and buccal swabs yielded 100% positive agreement and 100% negative agreement with expected results.
Analytical Sensitivity/Limit of Detection:
The lower limit of detection of this assay is 1.25 genome copies/mcL for urine and buccal swabs.
Precision:
Inter-assay and intra-assay precisions were 100%.
Specificity:
No sequences were identified that would result in cross-reactivity with the assay by in silico analysis. No cross-reactivity was detected in experiments testing a panel of nucleic acid extracts from more than 50 bacterial, fungal, and viral organisms causing similar disease or commonly found in urine or buccal swabs.
Reportable Range:
This is a qualitative assay, and the results are reported as either negative or positive for the mumps virus target.
Day(s) Performed
Monday through Friday
Report Available
1 to 3 daysSpecimen Retention Time
1 weekPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| MUMPU | Mumps Virus PCR, Urine | 86580-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 617824 | Mumps Virus PCR, Urine | 86580-8 |