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HelpTest Code MUMPR Mumps Virus, Molecular Detection, PCR, Buccal
Ordering Guidance
Polymerase chain reaction testing (this test) is recommended as the first-line test if a patient has symptoms of mumps (ie, fever, swollen salivary/parotid glands).
If serology has been performed and IgM-class antibodies against mumps are detected (MMPGM / Mumps Virus Antibody, IgM and IgG, Serum), this test should be ordered to confirm mumps infection.
Shipping Instructions
Specimens should be transported as soon as possible.
Specimen Required
Specimen Type: Buccal Swab
Supplies: Culturette (BBL Culture Swab) (T092)
Container/Tube: Sterile container with transport media
Specimen Volume: Entire collection
Collection Instructions:
1. Collect specimen by swabbing back and forth over mucosal surface around buccal cavity (the space near the upper rear molars between the cheek and the teeth) to maximize recovery of cells.
2. Swab must be placed into viral transport media (eg, M4-RT, M4, M5, Barthels FlexTrans Media or Jiangsu Transport Media)
Useful For
Rapid qualitative detection of mumps virus using buccal swab specimens
Reporting Name
Mumps Virus PCR, BuccalSpecimen Type
SwabSpecimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Swab | Refrigerated (preferred) | 7 days | |
| Frozen | 7 days |
Reject Due To
| E-swab, calcium alginate-tipped swab, wood swab, dry swab, or transport swab containing gel or charcoal additive | Reject |
| Throat swab | Reject |
Reference Values
Negative
Supportive Data
The following validation data support the use of this assay for clinical testing.
Accuracy:
Accuracy studies were performed by testing negative and positive (near the limit of detection) urine and buccal swab samples. Both urine and buccal swabs yielded 100% positive agreement and 100% negative agreement with expected results.
Analytical Sensitivity/Limit of Detection:
The lower limit of detection of this assay is 1.25 genome copies/mcL for urine and buccal swabs.
Precision:
Inter-assay and intra-assay precisions were 100%.
Specificity:
No sequences were identified that would result in cross-reactivity with the assay by in silico analysis. No cross-reactivity was detected in experiments testing a panel of nucleic acid extracts from more than 50 bacterial, fungal, and viral organisms causing similar disease or commonly found in urine or buccal swabs.
Reportable Range:
This is a qualitative assay, and the results are reported as either negative or positive for the mumps virus target.
Day(s) Performed
Monday through Friday
Report Available
1 to 3 daysSpecimen Retention Time
1 weekPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| MUMPR | Mumps Virus PCR, Buccal | 47532-7 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 617823 | Mumps Virus PCR, Buccal | 47532-7 |