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HelpTest Code MPCDS mSMART, Plasma Cell Proliferative Disorder, FISH, Bone Marrow
Specimen Required
Only orderable as part of a profile. For more information see MSMRT / Mayo Algorithmic Approach for Stratification of Myeloma and Risk-Adapted Therapy Report, Bone Marrow.
Specimen Type: Redirected bone marrow
Preferred: Yellow top (ACD)
Acceptable: Lavender top (EDTA) or green top (heparin)
Specimen Volume: 4 mL
Useful For
Aiding in the diagnosis of new cases of multiple myeloma or other plasma cell proliferative disorders as a part of a profile
Identifying prognostic markers based on the anomalies found
Testing Algorithm
This test is designed for diagnostic specimens from patients with multiple myeloma or other plasma cell proliferative disorders.
For diagnostic samples, all probes in the initial panel will be evaluated if sufficient plasma cells are identified. The initial panel includes testing for the following the probes listed:
17p-, TP53/D17Z1
1q gain, TP73/1q22
14q32 rearrangement, IGH break-apart
8q24.1 rearrangement, MYC break-apart
Based on the results from the initial panel, reflex testing may be performed to identify the following abnormalities using the probes listed:
t(11;14)(q13;q32), CCND1/IGH fusion
t(14;16)(q32;q23) IGH/MAF fusion
t(4;14)(p16.3;q32) FGFR3/IGH fusion
t(14;20)(q32;q12) IGH/MAFB fusion
t(6;14)(p21;q32) CCND3/IGH fusion
Hyperdiploidy will be evaluated and reported by flow cytometry as part of this evaluation and incorporated into the final interpretation. For samples with an unsuccessful flow evaluation for hyperdiploidy and with sufficient plasma cells, fluorescence in situ hybridization testing for the following abnormalities will be performed using the probes listed:
+3/+7, D3Z1/D7Z1
+9/+15, D9Z1/D15Z4
For specimens sent for follow-up testing after completion of initial testing, the following probes will be evaluated if sufficient plasma cells are identified:
17p-, TP53/D17Z1
1q gain, TP73/1q22
8q24.1 rearrangement, MYC break-apart
Based on the results from the initial follow-up panel, reflex testing may be performed to identify the following high- risk abnormalities that were originally identified in the diagnostic specimen, using the probes listed:
t(14;16)(q32;q23) IGH/MAF fusion
t(4;14)(p16.3;q32) FGFR3/IGH fusion
t(14;20)(q32;q12) IGH/MAFB fusion
If a diagnostic sample was uninformative for a probe set due to an insufficient number of plasma cells, attempts may be made to achieve results for the missing probe on a subsequent sample (if sufficient plasma cells are identified).
Appropriate ancillary probes may be performed at consultant discretion to render comprehensive assessment. Any additional probes will have the results included within the final report and will be performed at an additional charge.
Reporting Name
mSMART Eval, PCPDs, FISHSpecimen Type
Bone MarrowSpecimen Minimum Volume
2 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Bone Marrow | Ambient (preferred) | ||
| Refrigerated | |||
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Reference Values
Only orderable as part of a profile. For more information see MSMRT / Mayo Algorithmic Approach for Stratification of Myeloma and Risk-Adapted Therapy Report, Bone Marrow.
An interpretive report will be provided.
Supportive Data
Each probe was independently tested and verified on unstimulated peripheral blood and bone marrow specimens. Normal cutoffs were calculated based on the results of 25 normal specimens. Each probe set was evaluated to confirm the probe set detected the abnormality it was designed to detect.
Day(s) Performed
Monday through Friday
Report Available
7 to 10 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
88271 x 2, 88274, 88291-FISH Probe, Analysis, Interpretation; 1 probe set
88271 x 2, 88274-FISH Probe, Analysis; each additional probe set (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| MPCDS | mSMART Eval, PCPDs, FISH | 93357-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 606091 | mSMART Result Summary | 62357-9 |
| 606092 | mSMART Evaluation | 57802-1 |
| 606093 | Interpretation | 69965-2 |
| 606094 | Result Table | 93356-4 |
| 606095 | Result | 62356-1 |
| 606096 | Reason for Referral | 42349-1 |
| 606097 | Specimen | 31208-2 |
| 606098 | Source | 85298-8 |
| 606099 | Method | 85069-3 |
| 606100 | Additional Information | 48767-8 |
| 606101 | Disclaimer | 62364-5 |
| 606102 | Released By | 18771-6 |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| MPCDB | Probe, Each Additional (MPCDS) | No, (Bill Only) | No |