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HelpTest Code MEASU Measles Virus, Molecular Detection, PCR, Random, Urine
Ordering Guidance
This polymerase chain reaction (PCR) test should be ordered for individuals with suspected measles infection and symptoms such as rash, cough, fever, and conjunctivitis 6 to 14 days of rash onset.
This test is not recommended for patients 0 to 5 days after rash onset. A PCR test on a throat swab is recommended: see MEASR / Measles Virus, Molecular Detection, PCR.
If measles virus IgM antibody testing is positive (ROGM / Measles [Rubeola] Virus Antibody, IgM and IgG, Serum) this PCR test should be ordered to confirm the diagnosis of measles infection.
Additional Testing Requirements
This test should be ordered together with MEASR / Measles Virus, Molecular Detection, PCR, Throat, in patients at 6 to 14 days of rash onset to increase the sensitivity of detecting the measles virus.
Shipping Instructions
Specimens must be shipped at refrigerated temperature. Specimens received frozen will be rejected.
Specimen Required
Container/Tube: Sterile tube
Specimen Volume: 1 mL
Collection Instructions:
1. Collect a random urine specimen during the 6-to-14-day period after rash onset in a sterile urine tube.
2. No preservative.
Useful For
Identifying measles infection using random urine specimens
Reporting Name
Measles Virus PCR, UrineSpecimen Type
UrineSpecimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Urine | Refrigerated | 7 days |
Reject Due To
| Samples that have been heat inactivated Urine containing preservatives |
Reject |
Reference Values
Negative
Supportive Data
The following validation data support the use of this assay for clinical testing.
Accuracy:
Accuracy studies were performed by testing negative and positive (near the limit of detection) urine and upper respiratory swab samples. Urine yielded 88% positive agreement and 100% negative agreement with expected results. Upper respiratory swabs yielded 100% positive and 100% negative agreement with expected results.
Analytical Sensitivity/Limit of Detection:
The lower limit of detection of this assay is 2 genome copies/mcL for urine and 1 copy/mcL for throat swabs.
Precision:
Inter-assay and intra-assay precisions were 100%.
Specificity:
No sequences were identified that would result in cross-reactivity with the assay by in silico analysis. No cross-reactivity was detected in experiments testing a panel of nucleic acid extracts from greater than50 bacterial, fungal, and viral organisms causing similar disease or commonly found in urine or throat swabs.
Reportable Range:
This is a qualitative assay, and the results are reported as either "negative" or "positive" for the measles virus target.
Day(s) Performed
Monday through Friday
Report Available
1 to 3 daysSpecimen Retention Time
1 weekPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| MEASU | Measles Virus PCR, Urine | 86577-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 617822 | Measles Virus PCR, Urine | 86577-4 |