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HelpTest Code LFACX Cryptococcus Antigen with Reflex, Spinal Fluid
Useful For
Aiding in the diagnosis of cryptococcosis
This test should not be used as a test of cure or to guide treatment decisions.
This test should not be performed as a screening procedure for the general population.
Disease States
- Cryptococcosis
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| CLFA | Cryptococcus Ag Screen w/Titer, CSF | Yes | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| CLFAT | Cryptococcus Ag Titer, LFA, CSF | Yes | No |
| FGENC | Fungal Culture, CSF | Yes, (Order FGEN) | No |
Testing Algorithm
If result is positive, Cryptococcus antigen titer will be performed at an additional charge.
If Cryptococcus antigen titer is positive, then fungal culture will be performed at an additional charge.
Reporting Name
Cryptococcus Ag w/Reflex, LFA, CSFSpecimen Type
CSFSpecimen Required
Container/Tube: Sterile vial
Specimen Volume: 1 mL
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| CSF | Refrigerated (preferred) | 14 days | |
| Frozen | 14 days |
Reject Due To
| Gross hemolysis | Reject |
Reference Values
CRYPTOCOCCUS ANTIGEN SCREEN WITH TITER
Negative
Reference values apply to all ages.
CRYPTOCOCCUS ANTIGEN TITER, LFA
Negative
Reference values apply to all ages.
FUNGAL CULTURE
Negative
If positive, fungus will be identified.
Reference values apply to all ages.
Supportive Data
Cerebrospinal fluid (CSF) retrospective specimens (111) were tested in a blinded fashion by the IMMY Cryptococcus Antigen Lateral Flow Assay (LFA) and the Meridian Latex Agglutination (Meridian Bioscience Inc) assay within a 24-hour period. Of these 111 specimens, 45 were also tested by the Meridian Cryptococcus Antigen EIA also within a 24-hour period. Samples with discordant results after initial testing were repeated by both assays during the same freeze/thaw cycle. The results are summarized below in Table 1 and Table 2:
Table 1. Comparison of the IMMY LFA to the Meridian latex agglutination assay in CSF
|
Meridian latex agglutination |
||||
|
LFA IMMY |
|
Positive |
Negative |
Total |
|
Positive |
18 |
0 |
18 |
|
|
Negative |
0 |
93 |
93 |
|
|
Total |
18 |
93 |
111 |
|
Sensitivity: 100% (18/18); 95% CI: 81.2%-100%
Specificity: 100% (93/93); 95% CI: 96.0%-100%
Overall Percent Agreement: 100% (111/111); 95% CI: 99.0%-100%
Table 2. Comparison of the IMMY LFA to the Meridian Cryptococcus antigen EIA in CSF
|
|
Meridian EIA |
|||
|
LFA IMMY |
|
Positive |
Negative |
Total |
|
Positive |
12 |
0 |
12 |
|
|
Negative |
0 |
33 |
33 |
|
|
Total |
12 |
33 |
45 |
|
Sensitivity: 100% (12/12); 95% CI: 72.2%-100%
Specificity: 100% (33/33); 95% CI: 87.8%-100%
Day(s) Performed
Monday through Sunday
Report Available
Same day/1 to 35 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87899-Cryptococcus Ag Screen w/Titer, CSF
87899-Cryptococcus Ag Titer, LFA, CSF (as appropriate)
87102-Fungal Culture, CSF (as appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| LFACX | Cryptococcus Ag w/Reflex, LFA, CSF | 29896-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 62074 | Cryptococcus Ag Screen w/Titer, CSF | 29896-8 |
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.