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Test Code LCMSP Microsporidia species, Molecular Detection, PCR, Varies

Useful For

Detecting Enterocytozoon bieneusi and Encephalitozoon species in fecal and urine specimens to support the clinical diagnosis of microsporidiosis

Reporting Name

Microsporidia PCR

Specimen Type

Varies


Ordering Guidance


For specimens other than feces or urine, MTBS / Microsporidia Stain, Varies should be ordered since it will detect other microsporidia not targeted by this assay.



Necessary Information


Specimen source is required.



Specimen Required


Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Unpreserved feces

Supplies:

-Stool container, Small (Random), 4 oz (T288)

-Stool Collection Kit, Random (T635)

Container/Tube: Fecal container

Specimen Volume: 5 g

 

Specimen Type: Preserved feces

Supplies:

-ECOFIX Stool Transport Vial (Kit) (T219)

-Stool Collection Kit, Random (T635)

Container/Tube: ECOFIX preservative

Specimen Volume: 5 g

 

Specimen Type: Urine

Container/Tube: Sterile container

Specimen Volume: 5 mL

Collection Instructions: Mid-stream, clean-catch, suprapubic aspirates and catheterization collections are acceptable. Submit in a clean, sterile container free from preservatives. The first portion of the voided urine (first void) is also acceptable.


Specimen Minimum Volume

Feces: 1 g
Urine: 0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Ambient  7 days
  Frozen  7 days

Reject Due To

Urine containing preservatives
Prostate secretions and samples
Feces in preservatives other than ECOFIX		 Reject

Reference Values

Negative

Supportive Data

In a study using 205 clinical specimens and 254 spiked specimens, the Microsporidia polymerase chain reaction (PCR) assay had 90% to 100% sensitivity and 100% specificity in all specimen types accepted for this assay. The limit of detection is between 500 to 5000 target DNA copies/mcL of specimen. When 10-fold dilutions of fresh stool specimens containing Enterocytozoon bieneusi spores were tested by both PCR and microscopy, PCR showed significantly greater sensitivity, with the ability to detect Enterocytozoon bieneusi at 1 to 2 dilutions lower than microscopy.

Day(s) Performed

Tuesday through Friday

Report Available

2 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LCMSP Microsporidia PCR 94332-4

 

Result ID Test Result Name Result LOINC Value
MSPS Specimen Source 31208-2
36744 Encephalitozoon species 94333-2
36745 Enterocytozoon bieneusi 94334-0

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-Microbiology Test Request (T244)

-Gastroenterology and Hepatology Test Request (T728)