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HelpTest Code LAB5508 Herpes Simplex Virus (HSV), Molecular Detection, PCR, Blood
Ordering Guidance
If herpes simplex virus (HSV) is suspected in sources other than blood, order HSVPV / Herpes Simplex Virus (HSV), Molecular Detection, PCR, Varies.
If HSV is suspected in cerebrospinal fluid, order HSVC / Herpes Simplex Virus (HSV), Molecular Detection, PCR, Spinal Fluid.
Specimen Required
Container/Tube: Lavender top (EDTA)
Specimen Volume: 1 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Useful For
Aiding in the rapid diagnosis of disseminated disease due to herpes simplex virus (HSV)
Qualitative detection of HSV DNA
This test should not be used to screen asymptomatic patients.
Reporting Name
Herpes Simplex Virus, PCR, BSpecimen Type
Whole Blood EDTASpecimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole Blood EDTA | Refrigerated (preferred) | 7 days | |
| Frozen | 7 days |
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Reference Values
HERPES SIMPLEX VIRUS (HSV)-1
Negative
HERPES SIMPLEX VIRUS (HSV)-2
Negative
Supportive Data
Accuracy:
Thirty negative EDTA whole blood specimens were spiked with herpes simplex virus (HSV) 1 and HSV 2 plasmid control at the limit of detection (10 copies DNA target/microliter). The spiked specimens were run in a blinded fashion along with approximately 30 negative (non-spiked) specimens; 100% of the spiked specimens were positive and 100% of the non-spiked specimens were negative.
Analytical Sensitivity/Limit of Detection:
The lower limit of detection of this assay is 10 DNA target copies per microliter. This was established in anogenital swabs and confirmed in the matrix of EDTA whole blood.
Analytical Specificity:
No polymerase chain reaction signal was obtained from extracts of 30 bacterial, viral, and fungal isolates that could be found as normal flora in sites normally tested for this organism or that could cause similar symptoms.
Day(s) Performed
Monday through Sunday
Report Available
Same day/1 to 4 daysSpecimen Retention Time
1 weekPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87529 x 2
87529 (if appropriate for government payers)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| HSVPB | Herpes Simplex Virus, PCR, B | 93440-6 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 618330 | HSV 1 PCR, B | 93439-8 |
| 618331 | HSV 2 PCR, B | 93438-0 |
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.