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HelpTest Code LAB5477 Mycobacteria and Nocardia Culture, Varies
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
Mycobacterial CultureSpecimen Type
VariesNecessary Information
1. Specimen source is required.
2. Alert the laboratory if Mycobacterium genavense is suspected, as this species requires addition of mycobactin J to the culture medium for optimal growth and recovery.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Body fluid
Container/Tube: Sterile container
Specimen Volume: 1.5 mL
Specimen Type: Bone marrow
Container/Tube: Sterile container, or green top (lithium or sodium heparin)
Specimen Volume: Entire collection
Specimen Type: Gastric washing
Container/Tube: Sterile container
Specimen Volume: 10 mL
Collection Instructions: Neutralize specimen within 4 hours of collection with 100 mg of sodium carbonate per 5 to 10 mL of gastric wash.
Specimen Type: Respiratory
Sources: Bronchoalveolar lavage fluid, bronchial washing, sputum
Container/Tube: Sterile container
Specimen Volume: 3 mL
Collection Instructions:
1. Collect 3 respiratory specimens for acid-fast smears and culture in patients with clinical and chest X-ray findings compatible with tuberculosis.
2. These 3 specimens should be collected at 8 to 24-hour intervals (24 hours when possible) and should include at least 1 first-morning specimen.
Specimen Type: Stool
Supplies: Stool Collection Kit, Random (T635)
Container/Tube: Sterile container
Specimen Volume: 5 to 10 g
Specimen Type: Tissue
Container/Tube: Sterile container
Specimen Volume: 5 to 10 mm
Collection Instructions: Collect a fresh tissue specimen.
Specimen Type: Urine
Container/Tube: Sterile container
Specimen Volume: 20 to 50 mL
Collection Instructions: Collect a random urine specimen.
Fresh tissue or body fluid is the preferred specimen type instead of a swab specimen. Recovery of mycobacteria from swabs is generally very low yield.
Specimen Type: Swab
Sources: Wound, tissue, or body fluid
Container/Tube: Culture transport swab (noncharcoal) culturette, or Eswab
Specimen Volume: Adequate specimen
Collection Instructions:
1. Before collecting specimen, wipe away any excessive amount of secretion and discharge, if appropriate.
2. Obtain secretions or fluid from source with sterile swab.
3. If smear and culture are requested or both a bacterial culture and mycobacterial culture are requested, collect a second swab to maximize test sensitivity.
Reject Due To
| Blood or fixed tissue Specimen in viral transport medium (including but not limited to M4, M5, BD viral transport media, thioglycolate broth) Saliva Swab sources of respiratory fluids (eg, sputum) Swab sources of nasal, sinus, ear, mouth, throat, or scalp Wood shaft or charcoal swab Petri dish |
Reject |
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Refrigerated (preferred) | 7 days |
| Ambient | 7 days |
Specimen Minimum Volume
See Specimen Required
Special Instructions
Day(s) Performed
Monday through Sunday
Specimen Retention Time
Raw specimen 3 to 7 days; Isolates from positive cultures kept 1 year.Report Available
42 to 70 daysReference Values
Negative
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ISMY | ID by 16S Sequencing | No, (Bill Only) | No |
| RMALM | Id MALDI-TOF Mass Spec AFB | No, (Bill Only) | No |
| RTBSP | Id, Mtb Speciation, PCR | No, (Bill Only) | No |
| TBT | Concentration, Mycobacteria | No, (Bill Only) | No |
| TISSR | Tissue Processing | No, (Bill Only) | No |
| LCTB | Id, MTB complex Rapid PCR | No, (Bill Only) | No |
Useful For
Detection and identification of Mycobacterium species, Nocardia species, and other aerobic actinomycetes
Testing Algorithm
When this test is ordered, a reflex test may be performed at an additional charge.
The following algorithms are available:
CPT Code Information
87116-Mycobacterial Culture
87015-Mycobacteria Culture, Concentration (if appropriate)
87118-Id MALDI-TOF Mass Spec AFB (if appropriate)
87150-Id, Mtb Speciation, PCR (if appropriate)
87153-Mycobacteria Identification by Sequencing (if appropriate)
87176-Tissue Processing (if appropriate)
87150- Id, MTB complex Rapid PCR (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CTB | Mycobacterial Culture | 543-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| CTB | Mycobacterial Culture | 543-9 |
Forms
If not ordering electronically, complete, print, and send 1 of the following:
-Microbiology Test Request (T244)
-General Request (T239)
Supportive Data
The Bactec 460 and Bactec MGIT 960 systems were compared. A total of 1963 patient specimens were cultured, including 1519 respiratory tract specimens that required decontamination with sodium hydroxide and 444 sterile specimens that did not need to be decontaminated. A total of 168 cultures grew acid-fast bacilli in 1 or both systems (8.5% positivity rate). The contamination rate for positive respiratory tract specimens was 3.8% in the Bactec 460 and 7.9% in the MGIT. Contamination of sterile specimens was 6.3% in the Bactec 460 and 10.1% in the MGIT. Combined rates were 4.3% for the Bactec 460 and 8.4% for the MGIT. The overall recovery rates for mycobacterial species, excluding Mycobacterium gordonae, were 82.8%, 79.1%, and 78.4% for the Bactec 460, MGIT 960, and solid media, respectively. Recovery rates for the Bactec 460 and MGIT 960 were considered to be equivalent.