Sign in →

Test Code LAB535 Vitamin D 25 Hydroxy

Performing Laboratory

Asante Rogue Regional Medical Center

Specimen Minimum Volume

0.3 mL

Billing Code

2071827

Methodology

Chemiluminescence

Specimen Requirements

Yellow (Gold) top tube (SST)

Plain Red Top Tube

Allow to clot and centrifuge ASAP after collection.

1.0 mL serum, SST or plain red top tube.

Day(s) Test Set Up

Performed daily Monday – Friday.

Test Classification and CPT Coding

82306   Vitamin D; 25 hydroxy, includes fraction(s), if performed

Additional Information

Use:  The role of Vitamin D in bone and mineral metabolism
was recognized from its first identification as a factor that could
cure rickets.  However, Vitamin D is now recognized as a
prohormone that has multiple roles in maintaining optimal
health.  Vitamin D3 (cholecalciferol) and Vitamin D2
(ergocalciferol) are the most abundant forms of Vitamin D in the
body.  Vitamin D3 is synthesized in the skin from
7-dehydrocholesterol in response to sunlight.  The best
nutrition sources of D3 are oily fish, primarily salmon and
mackerel.  Vitamin D2’s nutrition sources are from some
vegetables, yeast and fungi.  The vegetarian diet is abundant
in Vitamin D2.

Total vitamin D (D2 and D3 expressed as a total result)

 

Vitamin D Status

25(OH) Vitamin D

Concentration Range

(ng/mL)

 

Deficient

< 20

Insufficient

20 to < 30

Sufficient

30 to 100

Toxic Level

> 100

 

Specimen Transport Temperature and Stability

Centrifuge within 2 hours of collection.  Stable 3 days room temp.  7 days 2-8 C. Freeze if testing delayed.

Performing Department

Chemistry

Reasons for Rejection

For assays employing antibodies, the possibility exists for interference by heterophile antibodies in the patient sample.  Patients who have been regularly exposed to animals or have received immunotherapy or diagnostic procedures utilizing immunoglobulins or immunoglobulin fragments may produce antibodies, e.g., HAMA, that interfere with immunoassays.  Additionally, other heterophile antibodies (e.g., human anti-sheep antibodies) may be present in patient samples.  Such interfering antibodies may cause erroneous results.  Carefully evaluate the results of patients suspected of having these antibodies.