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HelpTest Code LAB5338 Herpes Simplex Virus (HSV) Type 1- and Type 2-Specific Antibodies, IgG, Serum
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
HSV Types 1 and 2 Ab, IgG, SSpecimen Type
SerumSpecimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.6 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 14 days |
| Frozen | 14 days |
Specimen Minimum Volume
0.4 mL
Day(s) Performed
Monday through Friday
Specimen Retention Time
14 daysReport Available
Same day/1 to 2 daysReference Values
Negative
Useful For
Determining whether a patient has been previously exposed to herpes simplex virus (HSV) types 1 and 2
Distinguishing between infection caused by HSV types 1 and 2, especially in patients with subclinical or unrecognized HSV infection
This test should not be used to diagnose active or recent infection.
CPT Code Information
86695
86696
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| HSVG | HSV Types 1 and 2 Ab, IgG, S | 81621-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| HS1G | HSV Type 1 Ab, IgG, S | 51916-5 |
| HS2G | HSV Type 2 Ab, IgG, S | 43180-9 |
Method Description
The BioPlex 2200 HSV (herpes simplex virus)-1 and HSV-2 IgG assay uses multiplex flow immunoassay technology. Two different populations of dyed beads are each coated with glycoprotein G -based antigens associated with HSV types 1 or 2. Patient sample is combined with sample diluent and bead set reagent in a reaction vessel. The mixture is incubated at 37° C. After a wash cycle, antihuman IgG antibody conjugated to phycoerythrin (PE) is added to the mixture and incubated at 37° C. Excess conjugate is removed in another wash cycle, and the beads are resuspended in wash buffer. The bead mixture then passes through a detector where the identity of the dyed beads is determined by the fluorescence of the dyes, and the amount of antibody captured by the antigen is determined by the fluorescence of the attached PE. Raw data is calculated in relative fluorescence intensity. Three additional dyed beads, an internal standard bead, a serum verification bead, and a reagent blank bead are present in each reaction mixture to verify detector response, the addition of serum to the reaction vessel, and the absence of significant nonspecific binding in serum.(Package insert: BioPlex 2200 System HSV-1 and HSV-2 IgG. Bio-Rad Laboratories; Version 665-0533C_EN, 04/2019)
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| HS1G | HSV Type 1 Ab, IgG, S | No | Yes |
| HS2G | HSV Type 2 Ab, IgG, S | No | Yes |
Supportive Data
Accuracy:
To evaluate the accuracy of the BioPlex HSV assay, 505 prospective serum specimens were tested by enzyme immunoassay (EIA) (HerpeSelect, Focus Diagnostics) and the BioPlex HSV-1/2 IgG assay. Specimens that had discordant results after initial testing were repeated by both assays during the same freeze/thaw cycle.
Further discrepancies were evaluated by glycoprotein G type-specific Western blot (WB) at the University of Washington Virology laboratory.
The results are summarized in Tables 1 and 2 below:
Table 1. Comparison of the Bio-Rad BioPlex HSV-1 IgG assay to the HerpeSelect HSV-1 EIA using prospective serum specimens (n=505).
|
HSV-1 by BioPlex |
|
HSV-1 by HerpeSelect EIA |
|||
|
Positive |
Negative |
Equivocal |
Total |
||
|
Positive |
254 |
5(a) |
0 |
259 |
|
|
Negative |
2(b) |
240 |
1 |
243 |
|
|
Equivocal |
0 |
3 |
0 |
3 |
|
|
Total |
256 |
248 |
1 |
505 |
|
a. All 5 specimens were positive by WB
b. Both specimens were positive by WB
Sensitivity=99.2% (254/256); 95% CI (97.0, 99.9)
Specificity=96.8% (240/248); 95% CI (93.7, 98.5)
Overall percent agreement=97.8% (494/505); 95% CI (96.1, 98.8)
Table 2. Comparison of the Bio-Rad BioPlex HSV-2 IgG assay to the HerpeSelect HSV-2 EIA using prospective serum specimens (n=505).
|
HSV-2 by BioPlex |
|
HSV-2 by HerpeSelect |
|||
|
Positive |
Negative |
Equivocal |
Total |
||
|
Positive |
115 |
9(a) |
2 |
126 |
|
|
Negative |
1(b) |
376 |
0 |
377 |
|
|
Equivocal |
1 |
1 |
0 |
2 |
|
|
Total |
117 |
386 |
2 |
505 |
|
a. Two of 9 specimens were positive by WB; 2 of these 9 specimens were equivocal by WB.
b. This specimen was negative by WB.
Sensitivity=98.3% (115/117); 95% CI (93.6, 99.9)
Specificity=97.4% (376/386); 95% CI (95.2, 98.7)
Overall percent agreement=97.2% (493/505); 95% CI (95.4, 98.4)
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen: