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HelpTest Code LAB5338 Herpes Simplex Virus (HSV) Type 1- and Type 2-Specific Antibodies, IgG, Serum
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
HSV Types 1 and 2 Ab, IgG, SSpecimen Type
SerumSpecimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.6 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 14 days |
Specimen Minimum Volume
0.4 mL
Day(s) Performed
Monday through Saturday
Specimen Retention Time
14 daysReport Available
Same day/1 to 2 daysReference Values
Negative
Useful For
Determining whether a patient has been previously exposed to herpes simplex virus (HSV) types 1 and 2
Distinguishing between infection caused by HSV types 1 and 2, especially in patients with subclinical or unrecognized HSV infection
This test should not be used to diagnose active or recent infection.
CPT Code Information
86695
86696
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| HSVG | HSV Types 1 and 2 Ab, IgG, S | 81621-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| HS1G | HSV Type 1 Ab, IgG, S | 51916-5 |
| HS2G | HSV Type 2 Ab, IgG, S | 43180-9 |
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| HS1G | HSV Type 1 Ab, IgG, S | No | Yes |
| HS2G | HSV Type 2 Ab, IgG, S | No | Yes |
Supportive Data
Accuracy:
To evaluate the accuracy of the BioPlex HSV assay, 505 prospective serum specimens were tested by enzyme immunoassay (EIA) (HerpeSelect, Focus Diagnostics) and the BioPlex HSV-1/2 IgG assay. Specimens that had discordant results after initial testing were repeated by both assays during the same freeze/thaw cycle.
Further discrepancies were evaluated by glycoprotein G type-specific Western blot (WB) at the University of Washington Virology laboratory.
The results are summarized in Tables 1 and 2 below:
Table 1. Comparison of the Bio-Rad BioPlex HSV-1 IgG assay to the HerpeSelect HSV-1 EIA using prospective serum specimens (n=505).
|
HSV-1 by BioPlex |
|
HSV-1 by HerpeSelect EIA |
|||
|
Positive |
Negative |
Equivocal |
Total |
||
|
Positive |
254 |
5(a) |
0 |
259 |
|
|
Negative |
2(b) |
240 |
1 |
243 |
|
|
Equivocal |
0 |
3 |
0 |
3 |
|
|
Total |
256 |
248 |
1 |
505 |
|
a. All 5 specimens were positive by WB
b. Both specimens were positive by WB
Sensitivity=99.2% (254/256); 95% CI (97.0, 99.9)
Specificity=96.8% (240/248); 95% CI (93.7, 98.5)
Overall percent agreement=97.8% (494/505); 95% CI (96.1, 98.8)
Table 2. Comparison of the Bio-Rad BioPlex HSV-2 IgG assay to the HerpeSelect HSV-2 EIA using prospective serum specimens (n=505).
|
HSV-2 by BioPlex |
|
HSV-2 by HerpeSelect |
|||
|
Positive |
Negative |
Equivocal |
Total |
||
|
Positive |
115 |
9(a) |
2 |
126 |
|
|
Negative |
1(b) |
376 |
0 |
377 |
|
|
Equivocal |
1 |
1 |
0 |
2 |
|
|
Total |
117 |
386 |
2 |
505 |
|
a. Two of 9 specimens were positive by WB; 2 of these 9 specimens were equivocal by WB.
b. This specimen was negative by WB.
Sensitivity=98.3% (115/117); 95% CI (93.6, 99.9)
Specificity=97.4% (376/386); 95% CI (95.2, 98.7)
Overall percent agreement=97.2% (493/505); 95% CI (95.4, 98.4)
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-General Request (T239)