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HelpTest Code LAB5315 Bordetella pertussis and Bordetella parapertussis, Molecular Detection, PCR, Varies
Necessary Information
Specimen source is required.
Specimen Required
The high sensitivity of amplification by polymerase chain reaction (PCR) requires the specimen to be processed in an environment in which contamination of the specimen by Bordetella pertussis or Bordetella parapertussis DNA is unlikely.
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Nasopharyngeal swab
Supplies:
Culture Swab - Liquid Stuarts/Single Swab (NP Swab) (T515)
Container/Tube: Rayon swab with an aluminum shaft placed in transport medium such as a green-top nasopharyngeal swab (rayon mini-tip) with Stuart's media (no charcoal), or Stuart's media with charcoal, or Amies media with or without charcoal (Transwab Nasopharyngeal with Charcoal System).
Additional Information:
1. Swab transport containers without charcoal must contain a pledget saturated with either Stuart's or Amies liquid media. Clear semi-solid/solid media is gel and will be rejected.
2. Other swab or media types may be inhibitory to PCR testing and will be rejected.
Acceptable:
Specimen Type: Nasopharyngeal (not throat) aspirate/wash or nasal aspirate/wash
Container/Tube: Sterile container with a screw top cap (no transport media)
Specimen Volume: Entire collection
Useful For
Preferred diagnostic test for the detection of Bordetella pertussis or Bordetella parapertussis
This test is not recommended for screening asymptomatic individuals who may carry B pertussis or parapertussis.
This test is not recommended for follow up of patients previously diagnosed with pertussis (ie, as a test of cure).
Reporting Name
Bordetella, PCR, VariesSpecimen Type
VariesSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Refrigerated (preferred) | 7 days | |
| Ambient | 7 days | ||
| Frozen | 7 days |
Reject Due To
| Nose, nasal, or throat swab Calcium alginate or cotton-tipped swab Swab sent in gel transport medium, viral/universal transport medium, or Regan Lowe media ESwab Swabs with solid plastic shaft Dry swab |
Reject |
Reference Values
Not applicable
Supportive Data
The assay targets the multicopy insertion gene sequences, IS481 and IS1001, of Bordetella pertussis and Bordetella parapertussis, respectively. This assay was previously performed using analyte specific reagents from Roche Diagnostics(3); these reagents are no longer available. The assay was revalidated using probes and primers with the same sequence but provided by an alternate vendor. Performance of the new assay was then compared to the previous assay, which used the Roche analyte specific reagents, using 374 nasopharyngeal swabs and washings submitted for Bordetella testing. Fifty-four specimens were positive (48 Bordetella pertussis and 6 Bordetella parapertussis) and 314 specimens were negative by both assays. Five nasopharyngeal specimens were positive for Bordetella pertussis or Bordetella parapertussis by the new assay and negative by the old assay. One nasopharyngeal specimen was positive for Bordetella pertussis by the old assay but negative by the new assay. Overall, there was 98% (368/374) agreement between the 2 assays. Bordetella holmesii cannot be distinguished from Bordetella pertussis by the assay. The analytical sensitivity of the assay is 1 target/mcL for nasopharyngeal swabs and 10 targets/mcL for nasopharyngeal wash/aspirates.
Day(s) Performed
Monday through Friday, Sunday
Report Available
1 to 3 daysSpecimen Retention Time
3 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87798 x 2
87798 (if appropriate for government payers)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BPRPV | Bordetella, PCR, Varies | 90441-7 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| BPRS | Specimen source | 31208-2 |
| 618312 | Bordetella pertussis PCR | 43913-3 |
| 618313 | Bordetella parapertussis PCR | 42588-4 |
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.