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Test Code LAB527 Insulin
Performing Laboratory
Asante Rogue Regional Medical Center
Specimen Minimum Volume
0.5 mL EDTA plasma
Billing Code
2053163
Methodology
Chemiluminescence (CLIA)
Specimen Requirements
Specimen can be collected at any time
Fasting preferred
Day(s) Test Set Up
Monday through Sunday
Test Classification and CPT Coding
83525 - Insulin; total
Additional Information
Insulin, produced by the beta cells of the pancreas, regulates the uptake and utilization of glucose, and is also involved in the regulation of protein synthesis and triglyceride storage. Glucose elicits the release of insulin in two phases. Studies have shown the first phase, which represents stored insulin, begins 1 to 2 minutes after intravenous injection of glucose and ends within 10 minutes. The second phase depends on continuing insulin synthesis and release and lasts until normoglycemia has been restored, usually within 60 to 120 minutes. Patients with insulin-dependent diabetes exhibit minimal or no insulin response while those with non-insulin-dependent diabetes may preserve the second phase, but lack the first phase in normal pulsatile insulin secretion.
Performing Department
Chemistry
Reasons for Rejection
Hemolysis
Quantity not sufficient (QNS)
Lack of Two Patient Identifiers:
1-Patient’s First & Last name
2-Patient’s Date of Birth
Specimen Transport Temperature and Stability
Centrifuge within 2 hours of collection
Seperate into tightly capped storage tube, don't remove cap at any time
Stable 8 hours at room temperature
Stable 24 hours at 2° to 8° C
Freeze serum if testing is delayed