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HelpTest Code LAB5211 Cytomegalovirus (CMV) Molecular Detection, PCR, Varies
Ordering Guidance
For plasma specimens order CMVQN / Cytomegalovirus (CMV) DNA Detection and Quantification by Real-Time PCR, Plasma.
For lower respiratory specimens, order CMVLR / Cytomegalovirus (CMV) Molecular Detection, PCR, Lower Respiratory.
Necessary Information
1. Specimen source is required.
2. Source information must include main anatomical site of collection.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Body fluid
Sources: Spinal, pleural, peritoneal, ascites, pericardial, amniotic, or ocular
Container/Tube:
Preferred: Sterile, screwcap, 5-mL aliquot tube
Acceptable: Sterile container
Specimen Volume: 0.5 mL
Collection Instructions: Do not centrifuge.
Specimen Type: Upper respiratory tract fluid
Sources: Nasopharyngeal aspirate or washing
Container/Tube:
Preferred: Sterile, screwcap, 5-mL aliquot tube
Acceptable: Sterile container
Specimen Volume: 1.5 mL
Collection Instructions: Do not centrifuge.
Specimen Type: Swab
Sources: Dermal, eye, nasal, saliva, throat, or genital
Supplies:
-Culturette (BBL Culture Swab) (T092)
-M4-RT (T605)
Container/Tube: Multimicrobe media (M4-RT, M4, M5, Bartels, or Jiangsu) and ESwab or Culturette
Collection Instructions: Place swab back into multimicrobe media.
Specimen Type: Tissue
Sources : Brain, colon, kidney, liver, lung, etc.
Supplies: M4-RT (T605)
Container/Tube: Sterile container containing 1 mL to 2 mL of sterile saline or multimicrobe medium (M4-RT, M4, M5, Bartels, or Jiangsu)
Specimen Volume: Entire collection
Collection Instructions: Submit only fresh tissue.
Specimen Type: Urine
Container/Tube: Sterile container
Specimen Volume: 1 mL
Collection Instructions: Collect a random urine specimen.
Specimen Type: Bone marrow
Container/Tube: Lavender top (EDTA)
Specimen Volume: 0.5 mL
Collection Instructions: Send bone marrow in original tube. Do not aliquot.
Useful For
Rapid qualitative detection of cytomegalovirus (CMV) DNA
This test is not intended for the monitoring of CMV disease progression.
Reporting Name
Cytomegalovirus, PCRSpecimen Type
VariesSpecimen Minimum Volume
Ocular Fluid, Spinal Fluid, or Urine: 0.3 mL; Body Fluid (pleural, peritoneal, ascites, pericardial): See Specimen Required; Upper respiratory tract specimens: (Nasopharyngeal aspirate or washing): 1 mL; Tissue: 2 × 2-mm biopsy
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Refrigerated (preferred) | 7 days | |
| Frozen | 7 days |
Reject Due To
| Calcium alginate-tipped swab Wood swab Transport swab containing gel Dry/flocked ESwab Feces Paraffin blocks Breast milk Heat-inactivated specimens |
Reject |
Reference Values
Negative
Reference values apply to all ages.
Supportive Data
The following validation data support the use of this assay for clinical testing.
Accuracy:
A total of 200 prospective clinical samples (respiratory [n=72], urine [n=67], spinal fluid [n=25], fresh tissue [n=18], amniotic fluid [n=10], and bone marrow [n=8]) were submitted to our reference laboratory for cytomegalovirus (CMV) real-time polymerase chain reaction (PCR) (Roche analyte specific reagents, Roche Diagnostics). Respiratory samples included bronchoalveolar lavage (BAL) fluid (n=25), bronchial washing (n=40), nasal swab (n=4), tracheal secretions (n=2), and throat swab (n=1). Each sample was tested by 6 real-time PCR assays, and the results were compared to consensus reference standard (4 of 6 results being in agreement). The performance of the Us9 CMV real-time PCR (laboratory-developed test) is summarized in the Table:
Table. Performance of the Us9 CMV real-time PCR assay following testing of prospective clinical samples (n=200)
|
US9 CMV PCR |
Consensus result |
||||
|
Positive |
Negative |
Kappa |
Sensitivity |
Specificity |
|
|
Positive |
45 |
0 |
0.99 |
97.8 (87.6-99.9) |
100 (97.1-100) |
|
Negative |
1 |
154 |
|
|
|
Analytical Sensitivity/Limit of Detection:
To evaluate the analytical sensitivity, whole virus control (Acrometrix, Life Technologies) at a starting concentration of 500,000 copies/mL was used to generate a dilution panel. In brief, samples were diluted 1:2 in tris-EDTA buffer to a final concentration of 8 copies/mL. Each member of the dilution panel was then tested in triplicate, with the limit of detection (LOD) being defined as the highest dilution at which all replicates tested positive. The LOD was determined to be 122 copies/mL.(1)
Analytical Specificity:
No PCR signal was obtained from extracts of 44 bacterial and viral isolates including Epstein-Barr virus, herpes simplex virus, varicella-zoster virus, human herpes virus (HHV) 6, HHV7, HHV8, and parvovirus.
Day(s) Performed
Monday through Sunday
Report Available
Same day/1 to 4 daysSpecimen Retention Time
7 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87496
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CMVPV | Cytomegalovirus, PCR | 5000-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| CMVPS | Specimen Source | 31208-2 |
| 618969 | Cytomegalovirus PCR | 5000-5 |
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.