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HelpTest Code LAB5122 Mumps Virus Antibody, IgM and IgG, Serum
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
Mumps Ab, IgM and IgG, SSpecimen Type
SerumSpecimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Heat-inactivated specimen | Reject |
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 14 days |
Specimen Minimum Volume
0.9 mL
Day(s) Performed
MMPM: Tuesday
MPPG: Monday through Saturday
Specimen Retention Time
14 daysReport Available
1 to 7 daysReference Values
IgM:
Negative: Index value 0.00-0.79
Reference value applies to all ages.
IgG:
Vaccinated: Positive (≥1.1 AI)
Unvaccinated: Negative (≤0.8 AI)
Reference values apply to all ages.
Useful For
Diagnosis of mumps virus infection
Determination of postimmunization immune response of individuals to the mumps vaccine
Documentation of previous infection with mumps virus in an individual with no previous record of immunization to mumps virus
CPT Code Information
86735-Mumps, IgG
86735-Mumps, IgM
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| MMPGM | Mumps Ab, IgM and IgG, S | 77250-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| MUMP1 | Mumps Ab, IgM, S | 6478-2 |
| MUMG | Mumps Ab, IgG, S | 6476-6 |
| DEXM | Index Value | 25419-3 |
| DEXG5 | Mumps IgG Antibody Index | 25418-5 |
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| MMPM | Mumps Ab, IgM, S | Yes | Yes |
| MPPG | Mumps Ab, IgG, S | Yes | Yes |
Supportive Data
IgM:
The SeraQuest mumps IgM kit showed a sensitivity of 97.3% and a specificity of 96.6% when 160 specimens were tested in parallel with a reference method.(Package insert: SeraQuest)
IgG:
To evaluate the accuracy of the BioPlex Mumps IgG multiplex flow immunoassay (MFI), 500 prospective serum specimens were analyzed in a blinded fashion by the SeraQuest Mumps IgG enzyme immunoassay (EIA) and the BioPlex Mumps IgG assay. Specimens with discordant results after initial testing were repeated by both assays during the same freeze/thaw cycle. Further discrepancies were evaluated by the Mumps IgG VIDAS enzyme-linked fluorescent immunoassay (ELFA; bioMerieux, Inc). The results are summarized below:
|
|
SeraQuest Mumps IgG EIA |
|||
|
BioPlex Mumps IgG |
|
Positive |
Negative |
Equivocal |
|
Positive |
412 |
4(a) |
8 |
|
|
Negative |
3(b) |
48 |
3 |
|
|
Equivocal |
5 |
6 |
11 |
|
(a) All 4 of these samples tested positive by VIDAS Mumps IgG ELFA
(b) One of these 3 samples tested negative by the VIDAS Mumps IgG ELFA
Sensitivity: 98.1% (412/420); 95% Confidence Intervals (CI): 96.2%-99.1%
Specificity: 82.8% (48/58); 95% CI: 70.9%-90.6%
Overall Percent Agreement: 94.2% (471/500); 95% CI: 91.8%-96.0%
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.