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Test Code LAB239 Culture, Urine

Useful For

Use: To isolate and identify potentially pathogenic organisms causing urinary tract infection.

Performing Laboratory

Asante Rogue Regional Medical Center (ARRMC)

 

Performing Department

Microbiology

Specimen Required

Voided (clean catch), catheterized urine OR Sterile Urine Collections: Obtained by surgical intervention, nephrostomy, urostomy, cystoscopy or suprapubic bladder aspirates. 

 
Preferred >3 mL in BD Urine Culture Transport Tube

If < 3 mL, Sterile Collection Container 

 

Thoroughly instruct patient for proper collection of “clean catch” specimen. 

FEMALE PATIENT INSTRUCTIONS:

  1. Use the cleansing toilette to thoroughly clean the genital area before collection.
  2. To correctly do so, start by separating the skin folds around the urinary opening.  Using your supplied toilette, cleanse the opening and surrounding areas going from front to back.  This will help rule out cross-contamination.
  3. Uncap the specimen cup; be careful not to touch the inside of the cup or lid.
  4. While keeping the skin folds separated, start urinating into the toilet.  Once you have a steady flow, move the cup into position to catch the remainder of the urine.  DO NOT start collecting the urine until the stream is well established.
  5. Recap the cup, making sure the lid is on tight.
     

MALE PATIENT INSTRUCTIONS:

  1. If necessary, retract the foreskin.  Using the supplied toilette wipe, clean the penis by wiping the urinary opening.
  2. Uncap the specimen cup; be careful not to touch the inside of the cup or lid.
  3. Keep the foreskin retracted while urinating
  4. Begin the initial urination into the toilet.  Once you have a steady flow, move the cup into position to catch the remainder of the urine.  DO NOT start collecting the urine until the stream is well established.
  5. Recap the cup, making sure the lid is on tight.

 Apply the completed patient label to the specimen cup.

Specimen Minimum Volume

Preserved (BD Transport Tube): 3 mL (Fill Line on Tube)

Unpreserved (Sterile Container): 0.5 mL

Specimen Stability Information

Preserved (BD Transport Tube):

  • Stable 48 hrs room temperature

Unpreserved (Sterile Container):

  • Refrigerate 2-8◦C: Stable 24 hrs.
  • Unrefrigerated: Stable 2 hrs

Reject Due To

  • Lacking Two Patient Identifiers:
    • 1-Patient's First & Last name 
    • 2-Patient's Date of Birth
  • Specimen unrefrigerated for more than 2 hours from collection time
  • Refrigerated specimen >24 hours old (<12 hours preferred)
  • BD urine transport tube.>48 hour old
  • Improper collection
  • 24 hour, or pooled specimens
  • Urine from catheter bag

Day(s) Performed

Daily

Report Available:

Negative Cultures:

  • 18-24 hours Voided and Catheterized Specimens
  • 48 hours Sterile Collections

Positive Cultures: Varies based upon testing needed; minimum 48 hours

Method Name

 Quantitative Aerobic Culture; isolation and presumptive ID of isolates.

CPT Code Information

87086 – Culture, bacterial; quantitative colony count, urine

Billing Code

2030260
Reflex Testing:  May include; Definitive Identification (Bacterial); Sensitivity; Serotyping; Beta Lactamase; Dtest; ESBL