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HelpTest Code LAB1232584 Gastrointestinal Pathogen Panel, PCR, Feces
Useful For
Rapid detection of gastrointestinal infections caused by:
-Campylobacter species (Campylobacter jejuni/Campylobacter coli/Campylobacter upsaliensis)
-Clostridioides difficile toxin A/B
-Plesiomonas shigelloides
-Salmonella species
-Vibrio species (Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio cholerae)
-Vibrio cholerae
-Yersinia species
-Enteroaggregative Escherichia coli (EAEC)
-Enteropathogenic E coli (EPEC)
-Enterotoxigenic E coli (ETEC)
-Shiga toxin
-E coli O157
-Shigella/Enteroinvasive E coli (EIEC)
-Cryptosporidium species
-Cyclospora cayetanensis
-Entamoeba histolytica
-Giardia
-Adenovirus F 40/41
-Astrovirus
-Norovirus GI/GII
-Rotavirus A
-Sapovirus
This test is not recommended as a test of cure.
Special Instructions
Reporting Name
GI Pathogen Panel, PCR, FSpecimen Type
FecalOrdering Guidance
Infectious agent-based recommendations for testing:
If an infection with Vibrio species, including cholera is suspected, consider ordering VIBC/Vibrio Culture, Stool in conjunction with this test.
It is not recommended that the following tests be concomitantly ordered if this test is ordered:
-ROTA / Rotavirus Antigen, Feces
-LADV / Adenovirus, Molecular Detection, PCR, Varies
-GIAR / Giardia Antigen, Feces
-CRYPS / Cryptosporidium Antigen, Feces
-CYCL / Cyclospora Stain, Feces
-STL / Enteric Pathogens Culture, Feces
-CAMPC / Campylobacter Culture, Feces
-SHIGC / Shigella Culture, Feces
-SALMC / Salmonella Culture, Feces
-YERSC / Yersinia Culture, Feces
-E157C / Escherichia coli O157:H7 Culture, Feces
-STFRP / Shiga Toxin, Molecular Detection, PCR, Feces
-CDPCR / Clostridioides difficile Toxin, PCR, Feces
-LNORO / Norovirus PCR, Molecular Detection, Feces
Additional Testing Requirements
In some cases, there may be local public health requirements that impact Mayo Clinic Laboratories (MCL) clients and require additional testing on specimens with positive results from this panel. Clients should familiarize themselves with local requirements. MCL recommends clients retain an aliquot of each specimen submitted for this test to perform additional testing themselves, as needed. If necessary, see Interpretation for detailed information about how to obtain this testing.
Shipping Instructions
Specimen must arrive within 96 hours of collection.
Do not freeze. Testing will be canceled on specimens received frozen.
Specimen Required
Supplies: Culture and Sensitivity Stool Transport Vial (T058)
Container/Tube:
Preferred: Specific modified Cary Blair transport system; see Additional Information for acceptable collection media
Acceptable: Approved Cary Blair transport system (15 mL of non-nutritive transport medium containing phenol red as a pH indicator)
Specimen Volume: Representative portion of feces
Collection Instructions:
1. Collect fresh fecal specimen and submit 1 gram or 5 mL in container with transport medium.
2. Place feces in preservative within 2 hours of collection.
3. Submit preserved feces in original container. Do not aliquot.
4. If unpreserved specimens received, testing will be canceled.
Additional Information:
If collection media other than those listed is utilized, testing may be canceled. Media listed have been verified for use by Mayo Clinic Laboratories.
Modified Cary Blair media:
Preferred: Culture and Sensitivity Stool Transport Vial (T058)
Acceptable: Meridian Para-Pak C and S, Cardinal Health Culture and Sensitivity Stool transport Vial
Cary Blair media: Remel Cary Blair, Remel; Protocol Cary Blair
Specimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Fecal | Ambient (preferred) | 4 days | |
| Refrigerated | 4 days |
Reject Due To
| Unapproved commercial transport media (eg, AlphaTec Enteric Transport Medium [ETM], Para-Pak Enteric Plus, Medical Chemical Corporation C and S Transport Medium [MCC]) Copan FecalSwab/ESwab Products containing formalin (eg, Sodium Acetate-Acetic Acid Formalin fixative [SAF]; PolyVinyl Alcohol fixative [PVA]; EcoFix preservative) Swabs (eg, Cary Blair gel swab; Rectal swab Stool swab; Gel swab) Endoscopy specimen Unpreserved stool |
Reject |
Reference Values
Negative (for all targets)
Supportive Data
The BioFire FilmArray Gastrointestinal Panel is an FDA-cleared assay for testing Cary-Blair-preserved stool. A performance verification study of the FilmArray Gastrointestinal Panel was completed at Mayo Clinic (Rochester Minnesota).(1) Five hundred clinical stool specimens (retrospective/stored samples=270; prospective samples=230) were evaluated. Results were compared to a reference standard result, which was defined as an organism identified by routine culture, microscopy, or a consensus (2 out of 3) result obtained by molecular and/or antigen assays. Among 500 clinical stool samples, the assay showed greater than 90% agreement for all targets. Several targets, including Plesiomonas shigelloides, Cyclospora cayetanensis, Entamoeba histolytica, Vibrio species, and enterotoxigenic Escherichia coli did not have an adequate number of positive samples to rigorously assess the sensitivity of these targets.
In order to supplement the data derived from clinical samples, spiking studies were completed to evaluate the accuracy of all targets, including those that could not be analyzed by clinical specimens alone. This group included: Campylobacter species (n=4), Clostridioides difficile (n=4), Plesiomonas shigelloides (n=4), Salmonella species (n=4), Yersinia enterocolitica (n=4), Vibrio cholerae (n=4), enteroaggregative E coli (n=4), enteropathogenic E. coli (n=8), enterotoxigenic E coli (n=4), E coli O157 (n=4), Shigella species (n=8), Cryptosporidium species (n=4), Cyclospora cayetanensis (n=8), Entamoeba histolytica (n=4), Giardia lamblia (n=4), adenovirus 40/41 (n=4), norovirus (n=8), rotavirus A (n=4), sapovirus (n=4), and astrovirus (n=8). All targets demonstrated 100% agreement with the expected result during the spiking studies.
Specimen Retention Time
7 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87507
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| GIP | GI Pathogen Panel, PCR, F | 82195-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| SRCGI | Specimen Source | 31208-2 |
| 37081 | Campylobacter species | 82196-7 |
| 37082 | C. difficile toxin | 82197-5 |
| 37083 | Plesiomonas shigelloides | 82198-3 |
| 37084 | Salmonella species | 82199-1 |
| 37085 | Vibrio species | 82200-7 |
| 37086 | Vibrio cholerae | 82201-5 |
| 37087 | Yersinia species | 82202-3 |
| 37088 | Enteroaggregative E. coli (EAEC) | 80349-4 |
| 37089 | Enteropathogenic E. coli (EPEC) | 80348-6 |
| 37090 | Enterotoxigenic E. coli (ETEC) | 80351-0 |
| 37091 | Shiga toxin producing E. coli | 82203-1 |
| 37092 | Escherichia coli O157 serotype | 82204-9 |
| 37093 | Shigella/Enteroinvasive E. coli | 80350-2 |
| 37094 | Cryptosporidium species | 82205-6 |
| 37095 | Cyclospora cayetanensis | 82206-4 |
| 37096 | Entamoeba histolytica | 82207-2 |
| 37097 | Giardia | 82208-0 |
| 37098 | Adenovirus F40/41 | 82209-8 |
| 37099 | Astrovirus | 82210-6 |
| 37100 | Norovirus GI/GII | 82211-4 |
| 37101 | Rotavirus | 82212-2 |
| 37103 | Sapovirus | 82213-0 |
| 37262 | Interpretation | 59464-8 |
Testing Algorithm
The following algorithms are available:
Day(s) Performed
Monday through Sunday
Report Available
1 to 2 daysForms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Gastroenterology and Hepatology Test Request (T728)
-Microbiology Test Request (T244)