Home
HelpTest Code LAB1232526 Enterovirus, Molecular Detection, PCR, Varies
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
Enterovirus PCRSpecimen Type
VariesOrdering Guidance
This test will detect enterovirus but will not differentiate viruses in this family or provide serotyping information.
Necessary Information
1. Specimen source is required.
2. Source information should include main anatomical site of collection.
Specimen Required
Submit a raw clinical sample (not a culture isolate) for enterovirus testing.
Submit only 1 of the following specimens:
Specimen Type: Body fluid
Sources: Pericardial, peritoneal
Container/Tube: Sterile container
Specimen Volume: 0.5 mL
Collection Instructions: Do not centrifuge.
Specimen Type: Spinal fluid
Container/Tube: Sterile vial
Specimen Volume: 0.5 mL
Collection Instructions:
1. Submit specimen from collection vial 2.
2. Do not centrifuge.
Specimen Type: Swab
Supplies: Culturette (BBL Culture Swab) (T092)
Sources: Dermal, eye, rectal, genital, nasopharyngeal, oropharyngeal, throat, nasal, or urethral
Container/Tube: Multimicrobe media (M4-RT) or similar viral transport media (M4 or M5) and Eswab
Specimen Volume: Entire specimen
Collection Instructions:
1. Rectal swab must have no visible fecal matter
2. Place swab back into multimicrobe media (M4-RT, M4, or M5)
Specimen Type: Respiratory
Sources: Bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, pleural fluid, sputum, or tracheal aspirate
Container/Tube: Sterile container
Specimen Volume: 1.5 mL
Collection Instructions: Do not centrifuge.
Reject Due To
| Calcium alginate-tipped swab Wood swab Transport swab containing gel Heat-inactivated specimen |
Reject |
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Refrigerated (preferred) | 7 days | |
| Frozen | 7 days |
Specimen Minimum Volume
Body and Respiratory fluids: 0.5 mL; Spinal fluid: 0.3 mL; Swab: See Specimen Required
Special Instructions
Day(s) Performed
Monday through Sunday
Specimen Retention Time
7 daysReport Available
2 to 3 daysReference Values
Negative
Useful For
Aiding in diagnosing enterovirus infections
This test should not be used to screen asymptomatic patients.
Testing Algorithm
For more information see Meningitis/Encephalitis Panel Algorithm
CPT Code Information
87498
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| LENT | Enterovirus PCR | 93856-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| SRC68 | Specimen Source | 31208-2 |
| 80066 | Enterovirus PCR | 93856-3 |
Supportive Data
Accuracy/Diagnostic Sensitivity and Specificity:
Mayo Clinic Laboratories compared the detection of enteroviruses from spinal fluid by conventional tube cell culture (MCR-5) and by LightCycler polymerase chain reaction (PCR). Of 715 specimens tested, enteroviruses were detected in 65 (9%) by conventional cell culture and 82 (11%) by LightCycler PCR. Twenty-two of 82 (27%) were exclusively positive by PCR, whereas only 5 of 65 (8%) were exclusively positive by conventional cell cultures.
Supplemental Data (Spiking Studies):
To supplement the data above, 30 or more negative specimens of each specimen type (spinal fluid [CSF]/sterile body fluid, dermal/ocular/rectal swabs, plasma, and upper and lower respiratory specimens) were spiked with enterovirus culture control at approximately 10 to 50 targets/mcL (the approximate limit of detection). The spiked specimens were run in a blinded manner along with negative (non-spiked) specimens of each specimen type. Of the spiked specimens, 97% to 100% were positive, and 100% of the non-spiked specimens were negative. A total of 489 spiked and non-spiked specimens were tested.
Assay Inclusivity:
The assay detected all 64 members of an enterovirus panel, consisting of coxsackieviruses, polio viruses, echoviruses, and other enteroviruses. Importantly, the detection of parechovirus serotypes was found to be variable.
Analytical Specificity/Limit of Detection:
The lower limit of detection of this assay is approximately 10 to 50 RNA target copies/mcL. This was confirmed in all specimen types accepted for this assay.
Specificity:
The assay cannot reliably distinguish between enterovirus and rhinovirus. However, no cross-reactivity was observed when a specificity panel was tested, including influenza A/B, respiratory syncytial virus, parainfluenza virus, herpes simplex virus, Epstein-Barr virus, varicella zoster virus, and cytomegalovirus.
Reportable Range:
This is a qualitative assay, and results are reported as either negative or positive for targeted enterovirus RNA.
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.