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HelpTest Code LAB1232267 Hepatitis E Virus IgM Antibody Confirmation, Serum
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
HEV IgM Ab Confirmation, SSpecimen Type
Serum SSTNecessary Information
Date of collection is required.
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Serum gel
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL Serum
Collection Instructions:
1. Centrifuge blood collection tube per collection tube manufacturer's instructions (eg, centrifuge and aliquot within 2 hours of collection for BD Vacutainer tubes).
2. Aliquot serum into plastic vial.
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum SST | Frozen (preferred) | |
| Refrigerated | 7 days | |
Specimen Minimum Volume
Serum: 0.2 mL
Special Instructions
Day(s) Performed
Every other Friday
Specimen Retention Time
14 daysReport Available
1 to 21 daysReference Values
Negative
Useful For
Confirmation of reactive hepatitis E virus IgM antibody screening test results for the diagnosis of acute or recent (<6 months) hepatitis E infection
Testing Algorithm
For information see Hepatitis E: Diagnostic Testing Algorithm.
CPT Code Information
86790
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| HEVML | HEV IgM Ab Confirmation, S | 14212-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 61903 | HEV IgM Ab Confirmation, S | 14212-5 |
Method Description
Highly purified recombinant hepatitis E virus (HEV) antigens (O2N, O2C, and O3 from genotypes 1 and 3, and O2M from genotype 1) are fixed to nitrocellulose membrane test strips. The test strips are incubated with the diluted patient serum sample, allowing virus-specific human IgM antibodies to bind to the antigens present on the test strips. Unbound human antibodies are then washed away. In the second step, the strips are incubated with anti-human IgM antibodies coupled with horseradish peroxidase enzyme. Unbound conjugate antibodies are then washed away. Specifically bound human IgM antibodies are detected with a staining reaction catalyzed by the peroxidase. A dark band will appear on the strip at the corresponding point if a reaction has taken place.(Package insert: recomLine HEV IgG/IgM kit. Mikrogen Diagnostik; rev 10/2014)
Forms
If not ordering electronically, complete, print, and send 1 of the following:
-Gastroenterology and Hepatology Test Request (T728)
-Infectious Disease Serology Test Request (T916)
-Microbiology Test Request (T244)