Home
HelpTest Code LAB1232050 Adenovirus, Molecular Detection, PCR, Varies
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
Adenovirus PCRSpecimen Type
VariesNecessary Information
Specimen source is required.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Body fluid
Sources: Pleural, peritoneal, ascites, pericardial, or amniotic
Container/Tube: Sterile container
Specimen Volume: 0.5 mL
Collection Instructions: Do not centrifuge.
Specimen Type: Respiratory
Sources: Bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate
Container/Tube: Sterile container
Specimen Volume: 1 mL
Specimen Type: Spinal fluid
Container/Tube: Sterile vial
Specimen Volume: 0.5 mL
Collection Instructions: Do not centrifuge.
Specimen Type: Stool
Supplies: Stool Collection Kit, Random (T635)
Container/Tube: Sterile container
Specimen Volume: 1 g
Specimen Type: Swab
Supplies: M4-RT (T605)
Sources: Nasal, throat, respiratory, genital, or ocular
Container/Tube: Multimicrobe media (M4-RT) and Eswabs
Specimen Volume: Entire specimen
Collection Instructions: Place swab back into a multimicrobe media (M4-RT, M4, or M5).
Specimen Type: Tissue
Supplies: M4-RT (T605)
Container/Tube: Sterile container containing 1 mL to 2 mL of sterile saline or multimicrobe medium (M4-RT, M4, or M5)
Specimen Volume: Entire collection
Collection Instructions: Collect fresh tissue specimen.
Specimen Type: Urine
Container/Tube: Sterile container
Specimen Volume: 1 mL
Collection Instructions: Collect a random urine specimen.
Reject Due To
| Calcium alginate-tipped swab Wood swab Transport swab containing gel |
Reject |
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Refrigerated (preferred) | 7 days | |
| Frozen | 7 days |
Specimen Minimum Volume
Body fluid and respiratory specimens: 0.5 mL
Spinal fluid and urine: 0.3 mL
Stool: 0.5 g
Swab or Tissue: See Specimen Required
Day(s) Performed
Monday through Friday
Specimen Retention Time
1 weekReport Available
2 to 5 daysReference Values
Negative
Useful For
Aiding in the diagnosis of adenovirus infections
CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| LADV | Adenovirus PCR | 39528-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| SRC65 | Specimen Source | 31208-2 |
| 89074 | Adenovirus PCR | 39528-5 |
Supportive Data
The following support the use of this assay for clinical testing.
Accuracy/Diagnostic Sensitivity and Specificity:
A study of 715 clinical specimens compared shell vial culture and this polymerase chain reaction (PCR) assay. Included in the study were 288 swab specimens (nasal, throat, rectal, skin), 125 eye specimens, 221 respiratory specimens (bronchial washings, sputa, bronchioalveolar lavage, tracheal secretions), 56 fresh tissue specimens, 72 stools, 5 urines, and 27 body fluids/other specimens. Specimens were inoculated into culture tubes and examined for cytopathic effects over a period of 14 days, and subsequently assayed with this LightCycler (LC) assay. Comparison of cell culture with LC PCR yielded the following: total specimens positive by LC PCR was 83 (stool=7; respiratory=8; tissue=3; swabs=29; eye specimens=30; miscellaneous= 1 and urine=4) and total specimens positive by culture were 76 (stool=6; respiratory=7; tissue=3; respiratory swabs=28; eye specimens=29; and urine=2, miscellaneous = 1). Of the 83 total positive specimens, PCR detected ~10% more adenovirus infections compared with culture. This assay detected all 57 serotypes of adenovirus tested.
Supplemental Data (Spiking Studies):
To supplement the above data, 30 negative specimens of various types (spinal fluid, ocular, respiratory, stool, urine, and plasma) were spiked with adenovirus positive control plasmid at the limit of detection (approximately 10 targets/microliter). The 30 spiked specimens were run in a blinded manner with 30 negative (non-spiked) specimens. One hundred percent of the spiked specimens were positive, and 100% of the non-spiked specimens were negative.
Analytical Sensitivity/Limit of Detection:
The limit of detection for this assay is 10 targets per microliter in specimen matrix.
Analytical Specificity:
No PCR signal was obtained from extracts of 150 bacterial, viral, parasitic, and fungal isolates that could cause similar disease or could be found as normal flora in sites normally tested for this organism.
Precision:
Interassay precision was 100% and intra-assay precision was 100%.
Reportable Range:
This is a qualitative assay and results are reported as negative or positive for targeted adenovirus DNA.
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.