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HelpTest Code LAB1232047 Toxoplasma gondii, Molecular Detection, PCR, Varies
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
Toxoplasma gondii PCRSpecimen Type
VariesNecessary Information
Specimen source is required.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Amniotic fluid
Container/Tube: Sterile container
Specimen Volume: 0.5 mL
Collection Instructions: Do not centrifuge.
Specimen Type: Spinal fluid
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Container/Tube:
Preferred: 12 x 75-mm screw cap vial
Acceptable: Sterile vial
Specimen Volume: 0.5 mL
Collection Instructions: Do not centrifuge.
Specimen Type: Fresh tissue
Supplies:M4-RT (T605)
Container/Tube:
Preferred: Multi-microbe medium (eg, M4-RT)
Acceptable: Sterile container with 1 to 2 mL of sterile saline
Specimen Volume: Entire collection
Collection Instructions: Submit only fresh tissue in a sterile container containing 1 mL to 2 mL of sterile saline or multi-microbe medium (M4-RT, M4, or M5)
Specimen Type: Ocular fluid
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container: 12 x 75-mm screw cap vial
Specimen Volume: 0.3 mL
Collection Instructions:
1. Aliquot collected fluid into screw-cap vial. Do not submit ocular fluid in syringe.
2. Do not centrifuge or dilute the specimen.
Reject Due To
| Heat-inactivated specimen | Reject |
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Refrigerated (preferred) | 7 days | |
| Frozen | 7 days |
Specimen Minimum Volume
Amniotic Fluid, Ocular Fluid, Spinal Fluid: 0.3 mL
Tissue: 2 × 2 mm biopsy
Special Instructions
Day(s) Performed
Monday through Saturday
Specimen Retention Time
7 daysReport Available
Same day/1 to 4 daysReference Values
Negative
Useful For
Supporting the diagnosis of acute cerebral, ocular, disseminated, or congenital toxoplasmosis
This test should not be used to screen healthy patients.
Testing Algorithm
For more information see Meningitis/Encephalitis Panel Algorithm
CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| PTOX | Toxoplasma gondii PCR | 29904-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| SRC74 | Specimen Source | 31208-2 |
| 81795 | Toxoplasma gondii PCR | 29904-0 |
Supportive Data
Analytical Sensitivity/Limit of Detection:
The limit of detection for this assay is less than 5000 copies/mL in spinal fluid, tissue, ocular fluid, and amniotic fluid.
Analytical Specificity:
No polymerase chain reaction signal was obtained from extracts of 20 bacterial, parasitic, and viral isolates from similar organisms and from organisms commonly found in the specimen types tested.
Precision:
Intra-assay precision and interassay precision are 100%.
Reference Range:
The reference range is "Negative" for this assay.
Reportable Range:
This is a qualitative assay and results are reported as "Negative" or "Positive."
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.