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Test Code LAB1232034 JC Virus, Molecular Detection, PCR, Spinal Fluid

Important Note

Mayo code is JCPCR


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Preferred: 12 x 75-mm screw cap vial

Acceptable: Sterile screw cap vial

Container/Tube: Sterile vial

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge.


Useful For

Aiding in diagnosing progressive multifocal leukoencephalopathy due to JC virus

 

This test is not to be used as a diagnostic tool for Creutzfeldt-Jakob disease

 

This test is not recommended for screening asymptomatic patients

Testing Algorithm

For more information see Meningitis/Encephalitis Panel Algorithm.

Reporting Name

JC Virus, PCR, CSF

Specimen Type

CSF

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
CSF Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

  All specimens will be evaluated at Mayo Clinic Laboratories for test suitability

Reference Values

Negative

Supportive Data

The following data supports the use of this assay for clinical testing.

 

Accuracy:

Twenty-six negative cerebrospinal fluid (CSF) specimens were spiked with JC virus (JCV)-positive control plasmid at the limit of detection (approximately 10 targets/mcL). The 26 spiked specimens were run in a blinded manner with 14 negative (non-spiked) specimens. One hundred percent of the spiked specimens were positive, and 100% of the non-spiked specimens were negative.

 

Analytical Sensitivity/Limit of Detection:

The lower limit of detection of this assay is 10 DNA target copies per mcL in CSF.

 

Analytical Specificity:

No polymerase chain reaction (PCR) signal was obtained from the extracts of 15 viral isolates that may cause similar symptoms or be found in the CSF, including herpes simplex virus types 1 and 2, Epstein-Barr virus, cytomegalovirus, human herpesvirus (HHV)-6, HHV-7, HHV-8, enterovirus, mumps, adenovirus, BK virus, and Simian virus 40 (SV40).

 

Precision:

Interassay precision was 100% and intraassay precision was 100%.

 

Reference Range:

The reference range in CSF is "negative" for this assay.

 

Reportable Range:

This is a qualitative assay, and the results are reported as either negative or positive for targeted JCV DNA.

Day(s) Performed

Monday through Friday

Report Available

Same day/ 1 to 4 days

Specimen Retention Time

7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
JCPCR JC Virus, PCR, CSF 33295-7

 

Result ID Test Result Name Result LOINC Value
618305 JC Virus, PCR, CSF 33295-7

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.