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HelpTest Code LAB1231814 Bordetella pertussis Antibody, IgG, Serum
Useful For
Diagnosis of recent infection with Bordetella pertussis in patients with symptoms consistent with whooping cough for 2 or more weeks
This test should not be used in neonates, young infants or in children between the ages of 4 to 7 years as the routine childhood vaccine schedule may interfere with result interpretation.
This test should not be used as a test of cure, to monitor response to treatment, or to determine vaccine status.
Reporting Name
B. pertussis Ab, IgG, SSpecimen Type
SerumOrdering Guidance
This test should be ordered in patients with 2 or more weeks of symptoms consistent with whooping cough. Nucleic acid amplification testing for Bordetella pertussis should be used in patients who have been symptomatic less than 2 weeks; order BPRP / Bordetella pertussis and Bordetella parapertussis, Molecular Detection, PCR, Varies.
Specimen Required
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 30 days | |
| Frozen | 30 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
| Heat inactivated | Reject |
Reference Values
≥100 IU/mL (Positive)
40-<100 IU/mL (Borderline)
<40 IU/mL (Negative)
Reference values apply to all ages.
Supportive Data
Accuracy:
A total of 108 previously characterized serum samples (originally tested by Focus Diagnostics Inc.) were evaluated by the EuroImmun antipertussis toxin (PT) IgG EIA and the results are indicated below.
|
Comparison of the EuroImmun and Focus Diagnostics Bordetella pertussis PT EIAs |
|||
|
                      |
Focus Diagnostics PT EIA |
||
|
Positive |
Negative |
||
|
EuroImmun PT EIA |
Positive |
18 |
0 |
|
Negative |
0 |
77 |
|
|
Borderline(a) |
8(b) |
5(c) |
|
(a) Testing of a convalescent sample is recommended. Samples not included in positive and negative agreement calculations below.
(b) All 8 samples had low positive values by the Focus assay.
(c) All 5 samples were near the lower end of the borderline range for the EuroImmun ELISA.
Positive Agreement: 100% (18/18); 95% CI: 79.3%-100%
Negative Agreement: 100% (77/77); 95% CI: 94.3%-100%
Day(s) Performed
Thursday
Report Available
Same day/1 to 7 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86615
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BORDG | B. pertussis Ab, IgG, S | 42330-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| BIGG | B. pertussis IgG | 29659-0 |
| DEXBG | B.pertussis Value | 42330-1 |
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.