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Test Code LAB1231760 West Nile Virus, RNA, PCR, Molecular Detection, Serum

Important Note

Mayo's Test Code: WNVS


Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container: Sterile container

Specimen Volume: 0.5 mL

Collection Instructions:

1. Within 2 hours of collection centrifuge and aliquot serum into a sterile container.

2. Serum specimens not aliquoted from the serum gel collection tube into a sterile container will be rejected.


Useful For

Rapid testing for West Nile virus (WNV) RNA (lineage 1 and lineage 2) using serum specimens

 

An adjunctive test to serology for detection of early WNV infection (ie, first few days after symptom onset)

 

This test should not be used for screening asymptomatic individuals and should only be used to test patients with signs and symptoms of WNV disease.

Testing Algorithm

The following algorithms are available:

-Meningitis/Encephalitis Panel Algorithm

-Mosquito-borne Disease Laboratory Testing

Reporting Name

West Nile Virus RNA, PCR, Serum

Specimen Type

Serum

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

Gross hemolysis Reject
Heat-inactivated specimen Reject

Reference Values

Negative

Reference values apply to all ages.

Supportive Data

The following validation data supports the use of this assay for clinical testing.

 

Accuracy/Diagnostic Sensitivity and Specificity:

Accuracy studies were performed by testing negative clinical specimens with whole viral genomic RNA for lineages 1 and 2 near the limit of detection (LOD) and yielded greater than or equal to 97% sensitivity and specificity.

 

Analytical Sensitivity/LOD:

The lower LOD of this assay is 1 to 5 target copies/mcL of RNA extract for serum, urine, and spinal fluid and 27 to 60 copies/mcL for EDTA whole blood.

 

Precision:

Inter-assay and intra-assay precisions are 100%.

 

Specificity:

A panel of 42 organisms that can be found in the specimen types acceptable for this assay, as well as closely-related viruses (eg, dengue types 1-4, Japanese encephalitis virus, hepatitis E virus, Murray Valley encephalitis virus, St. Louis encephalitis virus, tick-borne encephalitis virus, yellow fever virus, Zika virus) and those that can cause a similar clinical syndrome were tested by this assay. No cross-reacting positive results were noted.

 

Reportable Range:

This is a qualitative assay, and the results are reported as either negative or positive for targeted West Nile virus.

Day(s) Performed

Monday through Friday

Report Available

Same day/1 to 5 days

Specimen Retention Time

1 week

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
WNVS West Nile Virus RNA, PCR, Serum 32361-8

 

Result ID Test Result Name Result LOINC Value
608436 West Nile Virus RNA, PCR, Serum 32361-8

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.