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HelpTest Code LAB1231755 Quantitative Lymphocyte Subsets: T, B, and Natural Killer (NK) Cells, Blood
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
QN Lymphocyte Subsets: T, B, and NKSpecimen Type
Whole Blood EDTAOrdering Guidance
This assay should not be used for diagnosing lymphocytic malignancies or evaluation of lymphocytosis of unknown etiology. For such cases, order LCMS / Leukemia/Lymphoma Immunophenotyping, Flow Cytometry, Varies, which includes a hematopathology review.
This assay can be used for absolute quantitation of B cells in patients with chronic lymphocytic leukemia.
While this assay can be used to follow patients on B-cell-depleting therapy, like rituximab (eg, Rituxan, Riabni) or ofatumumab (eg, Kesimpta), it may be more reasonable and financially viable to use CD20B / CD20 on B Cells, Blood (includes CD45, CD19 and CD20 markers).
Shipping Instructions
It is recommended that specimens arrive within 24 hours of collection. Collect and package specimen as close to shipping time as possible.
Necessary Information
Date and time of collection are required.
Specimen Required
Container/Tube: Lavender top (EDTA)
Specimen Volume: 3 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Additional Information: For serial monitoring, it is recommended that specimen collection be performed at the same time of day.
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole Blood EDTA | Ambient | 52 hours | PURPLE OR PINK TOP/EDTA |
Specimen Minimum Volume
1 mL
Special Instructions
Day(s) Performed
Monday through Sunday
Specimen Retention Time
4 daysReport Available
Same day/1 to 2 daysReference Values
The appropriate age-related reference values will be provided on the report.
Useful For
Serial monitoring of CD4 T-cell count in patients who are HIV-positive
Follow-up and diagnostic evaluation of primary immunodeficiencies, including severe combined immunodeficiency
Immune monitoring following immunosuppressive therapy for transplantation, autoimmunity, and other immunological conditions where such treatment is utilized
Assessment of immune reconstitution post-hematopoietic cell transplantation
Early screening of gross quantitative anomalies in lymphocyte subsets in infection or malignancies
Absolute quantitation of circulating B cells for diagnosis of patients with chronic lymphocytic leukemia as indicated in the 2008 International Workshop on Chronic Lymphocytic Leukemia guidelines
Testing Algorithm
For information see Newborn Screen Follow-up for Severe Combined Immunodeficiency Syndrome (SCID).
CPT Code Information
86355
86357
86359
86360
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TBBS | QN Lymphocyte Subsets: T, B, and NK | 80721-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 3321 | CD45 Total Lymph Count | 27071-0 |
| 3316 | % CD3 (T Cells) | 8124-0 |
| 3322 | CD3 (T Cells) | 8122-4 |
| 3319 | % CD4 (T Cells) | 8123-2 |
| 3325 | CD4 (T Cells) | 24467-3 |
| 3320 | % CD8 (T Cells) | 8101-8 |
| 3326 | CD8 (T Cells) | 14135-8 |
| 3318 | % CD19 (B Cells) | 8117-4 |
| 3324 | CD19 (B Cells) | 8116-6 |
| 4054 | % CD16+CD56 (NK cells) | 8112-5 |
| 4055 | CD16+CD56 (NK cells) | 20402-4 |
| 3327 | 4/8 Ratio | 54218-3 |
| 6657 | Comment | 80722-2 |
Disease States
- HIV infection