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HelpTest Code LAB1231737 Bartonella, Molecular Detection, PCR, Blood
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
Bartonella PCR, BSpecimen Type
Whole Blood EDTAOrdering Guidance
If this test result is negative and there is a strong suspicion of disease caused by these organisms, consider BART / Bartonella Antibody Panel, IgG and IgM, Serum and Warthin-Starry tissue stain (PATHC / Pathology Consultation) testing.
Specimen Required
The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Bartonella species DNA is unlikely.
Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Royal blue top (EDTA), pink top (EDTA), or sterile vial containing EDTA-derived aliquot
Specimen Volume: 1 mL
Collection Instructions: Send whole blood specimen in original tube (preferred).
Reject Due To
| Bone marrow | Reject |
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood EDTA | Refrigerated (preferred) | 7 days |
| Ambient | 7 days | |
| Frozen | 7 days |
Specimen Minimum Volume
0.5 mL
Special Instructions
Day(s) Performed
Monday through Sunday
Specimen Retention Time
1 weekReport Available
2 to 7 daysReference Values
Not applicable
Useful For
Aiding in the diagnosis of Bartonella infection when Bartonella DNA is expected to be present in blood, especially endocarditis
Testing Algorithm
For information see Infective Endocarditis: Diagnostic Testing for Identification of Microbiological Etiology.
CPT Code Information
87801
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BARTB | Bartonella PCR, B | 16275-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| SRC98 | Specimen Source | 31208-2 |
| 56056 | Bartonella PCR | 16275-0 |
Method Description
Bacterial nucleic acid is extracted from the specimen using the automated MagNA Pure instrument. The purified DNA is placed on the LightCycler instrument, which amplifies and monitors by fluorescence the development of target nucleic sequences after each polymerase chain reaction (PCR) cycle. A specific target sequence from Bartonella species is amplified and the resulting segment is detected using specific hybridization probes. Detection of the bartonella target is performed through melting curve analysis using the LightCycler software.(Cockerill FR, Uhl JR: Applications and challenges of real-time PCR for the clinical microbiology laboratory. In: Reischl U, Wittwer C, Cockerill F, eds. Rapid Cycle Real-Time PCR Methods and Applications. Springer-Verlag; 2002:3-27; Dumler JS, Carroll KC, Patel R: Bartonella. In: Carroll K, Pfaller M, Landry ML, et al, eds. Manual of Clinical Microbiology. 12th ed. ASM Press; 2019:893-904)
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.