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HelpTest Code KLISH Kappa and Lambda Light Chain mRNA, In Situ Hybridization (ISH) Technical Component Only
Advisory Information
This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.
Shipping Instructions
Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Specimen Required
Supplies: Immunostain Technical Only Envelope (T693)
Specimen Type: Tissue
Container/Tube: Immunostain Technical Only Envelope (T693)
Preferred: 5 Unstained positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.
Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block
Additional Information:
1. Information on accessing digital images of immunohistochemistry (IHC) stains and the manual requisition form can be accessed through this website: www.mayomedicallaboratories.com/test-info/ihc/index.html
2. Clients ordering stains using a manual requisition form will not have access to digital images.
3. Clients wishing to access digital images must place the order for IHC stains electronically. Information regarding digital imaging can be accessed through this website: www.mayomedicallaboratories.com/test-info/ihc/faq.html
Forms
If not ordering electronically, complete, print, and send an Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request Form (T763) (http://www.mayomedicallaboratories.com/it-mmfiles/ihc-and-ish-stain-request-form-mc0767-09a.pdf).
Useful For
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ISTOI | ISH Initial, Tech Only | No | No |
| ISTOA | ISH Additional, Tech Only | No | No |
Method Name
In Situ Hybridization
Reporting Name
Kappa/Lambda ISH, Tech OnlySpecimen Type
TECHONLYSpecimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| TECHONLY | Ambient (preferred) | |
| Refrigerated | ||
Reject Due To
|
Hemolysis |
NA |
|
Lipemia |
NA |
|
Icterus |
NA |
|
Other |
Wet/frozen tissue |
Clinical Information
Interpretation
The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request; contact 855-516-8404.
Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Cautions
No significant cautionary statements
Clinical Reference
2. Weiss LM, Movahed LA, Chen YY, et al: Detection of immunoglobulin light-chain mRNA in lymphoid tissues using a practical in-situ hybridization method. Am J Pathol 1990;137:979-988
3. Ruprai AK, Pringle JH, Angel CA, et al: Localization of immunoglobulin light-chain mRNA expression in Hodgkin's disease by in situ hybridization. J Pathol 1991;164:37-40
Method Description
In situ hybridization on sections of paraffin-embedded tissue.(Unpublished Mayo method)
Day(s) and Time(s) Performed
Monday through Friday
Analytic Time
1 daySpecimen Retention Time
Until staining is completePerforming Laboratory
Mayo Medical Laboratories in Rochester
Test Classification
This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
88365-TC, primary
88364-TC, if additional ISH
LOINC Code Information
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 70792 | Kappa/Lambda ISH, Tech Only | No LOINC Needed |