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HelpTest Code KKRP Kingella kingae, Molecular Detection, PCR, Varies
Useful For
Aiding in the diagnosis of Kingella kingae infection using tissue or synovial fluid specimens
Reporting Name
Kingella kingae PCRSpecimen Type
VariesNecessary Information
Specimen source is required.
Specimen Required
The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Kingella kingae DNA is unlikely.
Submit only 1 of the following specimens:
Specimen Type: Synovial fluid
Preferred: Lavender top (EDTA)
Acceptable: Pink top (EDTA), royal blue top (EDTA), sterile vial containing EDTA-derived aliquot, red clot tube (no anticoagulant), or sterile container
Specimen Volume: 0.5 mL
Collection Instructions: Send specimen in original tube (preferred).
Specimen Stability Information: Refrigerated (preferred) <7 days /Frozen <7 days
Specimen Type: Fresh tissue or biopsy
Sources: Bone, joint, synovium, heart valve, aorta, or endocardium
Container/Tube: Sterile container
Specimen Volume: Entire collection or 5 mm(3)- approximately the size of a pencil eraser
Collection Instructions:
1. Collect fresh tissue specimen.
2. Submit tissue only, do not add fluid to tissue
3. Refrigerate or freeze specimen.
Specimen Stability Information: Refrigerated (preferred) <7 days/ Frozen <7 days
Preferred Paraffin-embedded tissue block:
Specimen Type: Formalin-fixed, paraffin-embedded tissue block (FFPE)
Sources: Bone, joint, synovium, heart valve, aorta, or endocardium
Supplies: Tissue Block Container (T553)
Container/Tube: Tissue block
Collection Instructions: Submit a formalin-fixed, paraffin-embedded tissue block to be cut and returned.
Specimen Stability Information: Ambient (preferred)/Refrigerated
Acceptable Paraffin-embedded tissue block:
Specimen Type: Formalin-fixed, paraffin-embedded tissue block (FFPE)
Sources: Bone, joint, synovium, heart valve, aorta, or endocardium
Container/Tube: Sterile container for each individual cut section (scroll).
Collection Instructions: Perform microtomy and prepare five separate 10-micron sections. Each section (scroll) must be placed in a separate sterile container for submission.
Specimen Stability Information: Ambient (preferred)/Refrigerated
Specimen Minimum Volume
Fluid/fresh tissue or biopsy: See Specimen Required
Paraffin-embedded tissue block: Two 10-micron sections
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Varies | ||
Reject Due To
| Tissue in formalin, formaldehyde, or acetone Decalcified bone Bone marrow Slides |
Reject |
Reference Values
Not applicable
Supportive Data
This assay was validated by testing 30-spiked positive samples and 10-negative samples for each accepted sample type; fresh tissue, formalin-fixed paraffin-embedded tissue (FFPE), synovial fluid, and EDTA blood. No PCR inhibition was encountered. The assay was 100% sensitive and specific. The assay showed no cross-reactivity when tested with a panel of 67 bacterial isolates, including Kingella species other than kingae. The limit of detection (LOD) in fresh tissue and FFPE was 73.7 CFU/mcL. The LOD of synovial fluid was 1.3 CFU/mcL.
Day(s) Performed
Monday through Friday
Report Available
2 to 7 daysSpecimen Retention Time
1 weekPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| KKRP | Kingella kingae PCR | 65809-6 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| KKSRC | Specimen Source | 31208-2 |
| 48324 | Kingella kingae PCR | 65809-6 |
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244)with the specimen.