Test Code JAK2M JAK2 V617F Mutation Detection, Bone Marrow
Performing Laboratory
Mayo Clinic Laboratories in RochesterReporting Name
JAK2 V617F Mutation Detection, BMSpecimen Type
Bone MarrowShipping Instructions
Specimen must arrive within 7 days of collection.
Specimen Required
Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Yellow top (ACD)
Specimen Volume: 2 mL
Collection Instructions:
1. Invert several times to mix bone marrow.
2. Send bone marrow specimen in original tube. Do not aliquot.
Reject Due To
Gross hemolysis | Reject |
Moderately to severely clotted | Reject |
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Bone Marrow | Ambient (preferred) | 7 days | PURPLE OR PINK TOP/EDTA |
Refrigerated | 7 days | PURPLE OR PINK TOP/EDTA |
Specimen Minimum Volume
1 mL
Special Instructions
Day(s) Performed
Monday through Friday
Specimen Retention Time
Bone marrow: 2 weeks; Extracted DNA: 3 monthsReport Available
2 to 5 daysReference Values
An interpretive report will be provided.
Useful For
Aiding in the distinction between a reactive blood cytosis and a chronic myeloproliferative disorder using bone marrow specimens
Testing Algorithm
For information see Myeloproliferative Neoplasm: A Diagnostic Approach to Bone Marrow Evaluation.
CPT Code Information
81270-JAK2 (Janus kinase 2) (eg, myeloproliferative disorder) gene analysis, p.Val617Phe (V617F) variant
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
JAK2M | JAK2 V617F Mutation Detection, BM | 72333-8 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
39723 | JAK2 Result | 53761-3 |
31157 | JAK2 V617F Mutation Detection, BM | 72333-8 |
Supportive Data
Analytical sensitivity is determined at 0.06% (by dilution of a JAK2 V617F-positive cell line DNA into a negative cell line DNA).
Forms
1. Hematopathology Patient Information (T676)
2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.