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HelpTest Code HTLVC Human T-Cell Lymphotropic Virus Types 1 and 2 (HTLV-1/-2) Antibody Screen with Confirmation, Spinal Fluid
Ordering Guidance
This test is for spinal fluid specimens only. For serum specimens, order HTLVI / Human T-Cell Lymphotropic Virus Types I and II Antibody Screen with Confirmation, Serum.
Necessary Information
Date of collection is required.
Specimen Required
Collection Container/Tube: Sterile vial
Specimen Volume: 1 mL
Collection Instructions: Submit spinal fluid (CSF) specimen from collection vial number 2, 3, or 4 (preferred). If vial number is not provided, then only clear CSF without visible hemolysis or xanthochromia will be accepted for testing.
Useful For
Qualitative screening detection of human T-cell lymphotropic virus types 1 and 2 (HTLV-1/-2) specific antibodies with confirmation and differentiation between HTLV-1 and HTLV-2 infection
This test should not be used as a screening or confirmatory test for any specimen other than spinal fluid.
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| HTLLC | HTLV -1/-2 Ab Confirmation, CSF | Yes | No |
Testing Algorithm
If human T-cell lymphotropic virus types 1 and 2 (HTLV-1/-2) antibody result is reactive, then HTLV-1/-2 antibody confirmation by line immunoassay will be performed at an additional charge.
Reporting Name
HTLV-1/-2 Ab Screen, CSFSpecimen Type
CSFSpecimen Minimum Volume
0.6 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| CSF | Frozen (preferred) | 30 days |
| Refrigerated | 14 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Xanthochromia | Reject |
| Precipitated specimens | Reject |
| Heat-treated specimens | Reject |
Reference Values
Negative
Method Description
The Avioq HTLV-I/II Microelisa System is an enzyme-linked immunosorbent assay in which the solid phase (microwells) is coated with a purified human T-cell lymphotropic virus (HTLV) type 1viral lysate, a purified HTLV-2 viral lysate, and a recombinant HTLV-1 p21E antigen. With the addition of a diluted test sample containing antibodies to either HTLV-1 or HTLV-2, complexes are formed by the interaction of the antibodies in the sample and the solid phase antigens. Following incubation, the sample is aspirated, and the well is washed with buffer. Subsequently, antihuman immunoglobulin (goat) conjugated with horseradish peroxidase is added, which binds the antibody-antigen complex during a second incubation. Following a wash and incubation with TMB (tetramethylbenzidine) substrate, a blue color is produced. The enzyme reaction is stopped by the addition of a sulfuric acid solution, which changes the color to yellow. The amount of HTLV-1/-2 specific antibodies present in the sample is proportional to color development.(Package insert: Avioq HTLV-I/II Microelisa System. Avioq, Inc; 03/2024)
Day(s) Performed
Monday, Tuesday, Thursday, Friday
Report Available
1 to 4 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86790
86689 (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| HTLVC | HTLV-1/-2 Ab Screen, CSF | 22361-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 604934 | HTLV-1/-2 Ab Screen, CSF | 22361-0 |
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.