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Test Code HRTVC Heartland Virus, RNA, Molecular Detection, PCR, Spinal Fluid


Ordering Guidance


Patients with a history of symptoms for more than 1 week may be negative by molecular tests (ie, real-time polymerase chain reaction) and may require serologic testing, which is available through the Centers for Disease Control and Prevention.



Specimen Required


Container/Tube: Sterile vial

Specimen Volume: 1 mL

Collection Instructions:

1. Send specimen from collection vial 2.

2. Do not centrifuge or heat inactivate.


Useful For

Aiding in the diagnosis of central nervous system infection caused by Heartland virus using spinal fluid specimens

Testing Algorithm

For more information see Meningitis/Encephalitis Panel Algorithm.

Reporting Name

Heartland Virus, PCR, CSF

Specimen Type

CSF

Specimen Stability Information

Specimen Type Temperature Time Special Container
CSF Refrigerated (preferred) 14 days
  Frozen  14 days
  Ambient  24 hours

Reject Due To

Heat-inactivated specimen Reject

Reference Values

Negative

Supportive Data

The following validation data supports the use of this assay for clinical testing.

 

Accuracy/Diagnostic Sensitivity and Specificity:

Heartland virus (HRTV) accuracy results produced greater than or equal to 90% positive, negative, and overall agreement among 70 total contrived samples for each matrix (cerebrospinal fluid [CSF] and serum).

 

Analytical Sensitivity/Limit of Detection:

The HRTV reverse transcription polymerase chain reaction (RT-PCR) assay analytical sensitivity was established and confirmed at 2.5 genome copies/microliter of RNA extract (2500 genome copies/mL) in both CSF and serum.

 

Precision:

Greater or equal to 95% qualitative concordance among test replicates for HRTV inter-assay and intra-assay reproducibility.

 

Specificity:

Panel of 13 organisms with similar phylogenetic association, clinical presentation, vector/transmission mode and/or sample type were tested by the HRTV RT-PCR assay and did not show any amplification. Additionally, NCBI BLAST searches of HRTV assay forward/reverse primers and PCR amplicon sequences against a panel of 82 organisms causing similar diseases or commonly found in CSF or serum were also used to determine potential cross-reactivity of notable organisms. The data indicate that HRTV RT-PCR assay will not detect any organisms other than Heartland virus.

 

Reportable Range:

This is a qualitative assay, and the results are reported as either negative or positive for targeted Heartland virus.

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 to 5 days

Specimen Retention Time

7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HRTVC Heartland Virus, PCR, CSF 94183-1

 

Result ID Test Result Name Result LOINC Value
620056 Heartland Virus, PCR, CSF 94183-1

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.