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HelpTest Code HPCRP Helicobacter pylori with Clarithromycin Resistance Prediction, Molecular Detection, PCR, Varies
Infectious
Ordering Guidance
This test uses isolates of Helicobacter pylori for testing. If testing directly from feces is desired, order HPFRP / Helicobacter pylori with Clarithromycin Resistance Prediction, Molecular Detection, PCR, Varies.
Additional Testing Requirements
1. If identification testing is needed; also order IDENT / Organism Referred for Identification, Aerobic Bacteria.
2. If susceptibility testing is needed; also order ZMMLS / Antimicrobial Susceptibility, Aerobic Bacteria, MIC, Varies.
Shipping Instructions
1. For shipping information see Infectious Specimen Shipping Guidelines.
2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance, if appropriate.
Necessary Information
Organism identification and specimen source are required.
Specimen Required
Supplies: Infectious Container, Large (T146)
Container/Tube: Agar slant or other appropriate media
Specimen Volume: Isolate
Collection Instructions:
1. Perform isolation of Helicobacter pylori in culture.
2. H pylori isolate must be submitted in pure culture. Do not submit mixed cultures.
Useful For
Assessing pure isolates of Helicobacter pylori to predict clarithromycin resistance or susceptibility
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| PCRID | Identification by PCR | No, (Bill Only) | No |
Testing Algorithm
When this test is ordered, the reflex test may be performed at an additional charge.
For more information see Helicobacter pylori Diagnostic Algorithm.
Special Instructions
Reporting Name
H pylori + Clarithro Resist PCRSpecimen Type
VariesSpecimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | ||
| Frozen | |||
| Refrigerated | |||
Reject Due To
| Agar plate ESwab Port-a-Cult |
Reject |
Reference Values
Not applicable
Supportive Data
During laboratory verification studies, 111 isolates of Helicobacter pylori (grown on Columbia agar with 5% sheep blood) with previous clarithromycin phenotypic susceptibility testing performed and which had undergone partial 23S ribosomal RNA gene sequencing were studied. This test matched phenotypic testing for 106/111 isolates (95.5% categorical agreement); a major error rate of 8.7% (2/23) and very major error rate of 3.4% (3/88) were observed. The assay results perfectly matched partial 23S ribosomal RNA gene sequencing data, including that performed on the 5 discordant isolates.
A subset of 45 of the isolates (including 1/5 isolates demonstrating a discordant result in the earlier study) were re-grown on tryptic soy agar with 5% sheep blood and re-assayed with this assay. The assay matched phenotypic testing in 44/45 isolates (97.8% categorical agreement); a major error rate of 0% and very major error rate of 3% (1/33) were observed. The polymerase chain reaction assay perfectly matched partial 23S ribosomal RNA gene sequencing data, including that performed on the single discordant isolates.
Day(s) Performed
Monday through Friday
Report Available
3 to 4 daysSpecimen Retention Time
30 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87150
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| HPCRP | H pylori + Clarithro Resist PCR | 88509-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| HPS2 | Specimen Source | 31208-2 |
| HPORG | Organism Identified by Client | In Process |
| 608005 | Helicobacter pylori Result | 49101-9 |
| 608006 | Clarithromycin Resistance Result | 88509-5 |
Forms
If not ordering electronically, complete, print, and send Gastroenterology and Hepatology Test Request (T728) with the specimen