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HelpTest Code ESBLS Extended-Spectrum Beta-Lactamase-Producing Gram-Negative Bacteria Surveillance Culture, Feces
Shipping Instructions
Specimen must arrive within 96 hours of collection.
Specimen Required
Patient Preparation: Do not use barium or bismuth before specimen collection.
Supplies: Culture and Sensitivity Stool Transport Vial (T058)
Specimen Type: Preserved feces
Container/Tube: Cary-Blair or modified Cary-Blair transport system is required.
Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of nonnutritive transport medium containing phenol red as a pH indicator. Submit sample in original Cary Blair medium container (not an aliquot of Cary Blair medium).
Specimen Volume: Representative portion of feces; 1 gram or 5 mL
Collection Instructions:
1. Collect fresh fecal specimen and place 1 gram or 5 mL in container with transport medium.
2. Place feces in preservative within 2 hours of collection.
3. Place vial in a sealed plastic bag.
Useful For
Screening for colonization of extended-spectrum beta-lactamase (ESBL)-producing organisms in stool
Screening fecal microbiota transplant donor feces for ESBL-producing organisms.
This test is not intended for medicolegal use.
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| RMALD | Ident by MALDI-TOF mass spec | No, (Bill Only) | No |
| CHESB | ESBL SUS Test Charge | No, (Bill Only) | No |
Testing Algorithm
When this test is ordered, the reflex tests may be performed at an additional charge. All bacterial isolates tested, to rule in or out as a potential extended-spectrum beta-lactamase (ESBL)-producing pathogen, will be billed for work performed and resources consumed. The following Enterobacterales will be tested for ESBL production: Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, and Proteus mirabilis.
Reporting Name
ESBL GNB Surveillance Culture, FSpecimen Type
FecalSpecimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Fecal | Ambient (preferred) | 4 days | |
| Refrigerated | 4 days |
Reject Due To
| Products containing formalin SAF fixative PVA fixative EcoFix preservative Endoscopy specimen Processed stool |
Reject |
Reference Values
Negative for extended-spectrum beta-lactamase-producing organisms
Positive for extended-spectrum beta-lactamase-producing organisms
Supportive Data
Thiry-two gram-negative bacilli (22 confirmed extended-spectrum beta-lactamase [ESBL]-producing isolates and 10 non ESBL-producing isolates with resistance to either ceftriaxone or ceftazidime) were evaluated. Recovery of ESBL-producing isolates with vancomycin, amphotericin B, ceftazidime, and clindamycin (VACC) media was 100%. Of the non ESBL-producing isolates, 70% (7/10) were inhibited while 30% grew on VACC but tested negative with ESBL confirmatory disk testing. This finding aligns with the package insert information for VACC media.
Day(s) Performed
Monday through Sunday
Report Available
4 to 6 daysSpecimen Retention Time
7 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87081-ESBL GNB surveillance culture
87077-Ident by MALDI-TOF mass spec (if appropriate)
87184-ESBL SUS test charge (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| ESBLS | ESBL GNB Surveillance Culture, F | 102121-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| ESBLS | ESBL GNB Surveillance Culture, F | 102121-1 |
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.