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HelpTest Code DNSAG Dengue Virus NS1 Antigen, Serum
Useful For
Aiding in the diagnosis of dengue virus infection
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| DENS1 | Dengue NS1 Ag, S | No | Yes |
| DNAGI | Dengue Ag Interpretation | No | Yes |
Reporting Name
Dengue Virus NS1 Ag, SSpecimen Type
SerumSpecimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Specimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 14 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
Reference Values
Negative
Reference values apply to all ages.
Supportive Data
The presence of nonstructural protein 1 (NS1) antigen overlaps with the dengue virus (DV) viremic phase for the first 4 to 5 days following infection and therefore, the performance characteristics of the InBios DV NS1 enzyme-linked immunosorbent assay (ELISA) were compared to the Focus Diagnostics DV real-time polymerase chain reaction (RT-PCR), which detects RNA from all 4 DV serotypes. Seventy-seven serum samples previously evaluated by the Focus Diagnostics RT-PCR assay were also tested by the InBios DV NS1 ELISA and the results are compared in the Table. Discordant samples were also tested by the PlateliaTM NS1 Ag enzyme immunoassay (EIA) (BioRad Laboratories).
Table. Comparison of the InBios NS1 EIA to RT-PCR for DV detection
|
InBios DV NS1 EIA |
Focus Diagnostics DV RT-PCR |
|
|
|
Positive |
Negative |
|
Positive |
24 |
7(b) |
|
Negative |
1(a) |
43 |
|
Equivocal |
0 |
2(c) |
a. This sample was negative by the Platelia NS1 EIA
b. Five samples were also positive by the Platelia NS1 EIA
c. One sample was negative, and one sample was indeterminate by the Platelia NS1 EIA
Sensitivity: 96% (24/25); 95% CI: 79.1%-100%
Specificity: 82.7% (43/52); 95% CI: 70.1%-90.9%
Overall Agreement: 87.1% (67/77); 95% CI: 77.6%-93%
Day(s) Performed
Tuesday
Report Available
Same day/1 to 7 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86790
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| DNSAG | Dengue Virus NS1 Ag, S | 75377-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| DENS1 | Dengue NS1 Ag, S | 75377-2 |
| DNAGI | Dengue Ag Interpretation | 69048-7 |
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.