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HelpTest Code DLAC D-Lactate, Plasma
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
D-Lactate, PSpecimen Type
Plasma NaFl-KOxOrdering Guidance
Urine is the preferred specimen for D-lactate determination, order DLAU / D-Lactate, Urine.
For determination of L-lactate (lactic acid), order LACS1 / Lactate, Plasma
Specimen Required
Supplies: Sodium Fluoride/Potassium Oxalate Tube, 2 mL (T275)
Collection Container/Tube:
Preferred: Sodium fluoride/potassium oxalate tube
Acceptable: Green top (sodium heparin)
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL plasma
Collection Instructions: Centrifuge and aliquot plasma into a plastic vial.
Note: If collecting in sodium heparin tubes, centrifugation must occur within one hour of collection.
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Plasma NaFl-KOx | Frozen (preferred) | 91 days |
| Ambient | 91 days | |
| Refrigerated | 91 days |
Specimen Minimum Volume
Plasma: 0.15 mL
Special Instructions
Day(s) Performed
Monday, Thursday
Specimen Retention Time
1 monthReport Available
3 to 6 daysReference Values
≤0.25 mmol/L
Useful For
As an adjunct to urine D-lactate (preferred) for the diagnosis of D-lactate acidosis
CPT Code Information
83605
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| DLAC | D-Lactate, P | 14045-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 8878 | D-Lactate, P | 14045-9 |
Method Description
Plasma is spiked with a mixture of internal standards and evaporated. The dry residue is derivatized with diacetyl-L-tartaric anhydride to form the diastereomeric molecules, then acidified and extracted with ethyl acetate. After evaporation, the dry residue is again derivatized to form trimethylsilyl esters at the carboxylic acid moiety. Specimens are then analyzed by capillary gas chromatography mass spectrometry selected ion monitoring using positive chemical ionization, with chromatographic separation of the L,L- and D,L-diastereoisomers of derivatized lactate, corresponding to L-Lactate and D-Lactate, respectively.(Ding X, Lin S, Weng H, Liang J. Separation and determination of the enantiomers of lactic acid and 2-hydroxyglutaric acid by chiral derivatization combined with gas chromatography and mass spectrometry. J Sep Sci. 2018;41(12):2576–2584)