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HelpTest Code DENVP Dengue Virus Antibody/Antigen Panel, Serum
Useful For
Aiding in the diagnosis of dengue virus infection by detection of IgM and IgG antibodies and the nonstructural protein 1 (NS1)
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| DENG | Dengue Virus Ab, IgG, S | No | Yes |
| DENM | Dengue Virus Ab, IgM, S | No | Yes |
| DENS1 | Dengue NS1 Ag, S | Yes, (DNSAG) | Yes |
| INT69 | Dengue Interpretation | No | Yes |
Reporting Name
Dengue Virus Ab/Ag Panel, SSpecimen Type
SerumSpecimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Specimen Minimum Volume
0.8 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 14 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
| Heat-inactivated specimen | Reject |
Reference Values
IgG: Negative
IgM: Negative
NS1: Negative
Reference values apply to all ages.
Supportive Data
A total of 200 prospective serum samples submitted for dengue virus (DV) IgM and IgG testing by the Focus Diagnostics DV IgM and IgG enzyme immunoassays (EIA) were also tested by the InBios IgM and IgG DV assays. The results were compared and the data summarized in Tables 1 and 2.
Table 1. Comparison of the InBios and Focus (Quest) Diagnostics DV IgM EIA
|
InBios DV IgM EIA |
Focus (Quest) Diagnostics DV IgM EIA |
|
|
Positive |
Negative |
|
|
Positive |
14 |
0 |
|
Negative |
1 |
184 |
|
Equivocal |
1 |
0 |
Sensitivity: 87.5% (14/16); 95% CI 62.7%-97.7%
Specificity: 100% (184/184); 95% CI 97.5%-100%
Agreement: 99% (198/200); 95% CI 96.1%-99.9%
Table 2. Comparison of the InBios and Focus (Quest) Diagnostics DV IgG EIA
|
InBios DV IgG EIA |
Focus (Quest) Diagnostics DV IgG EIA |
|
|
|
Positive |
Negative |
|
Positive |
34 |
0 |
|
Negative |
0 |
164 |
|
Equivocal |
2 |
0 |
Sensitivity: 94.4% (34/36); 95% CI 80.9%-99.4%
Specificity: 100% (164/164); 95% CI 97.2%-100%
Agreement: 99% (198/200); 95% CI 96.1%-99.9%
An additional 42 serum samples positive for IgG-class antibodies to West Nile virus (n=24), St. Louis encephalitis virus (n=9) and California (LaCrosse) virus (n=9) were also tested by the InBios DV IgG EIA and the data are summarized below in Table 3.
Table 3. Cross-reactivity of the InBios DV IgG EIA with antibodies to West Nile virus, St. Louis encephalitis virus, and California (LaCrosse) virus
|
InBios DV IgG EIA |
West Nile virus IgG positive |
St. Louis encephalitis virus positive |
California (LaCrosse) virus positive |
|
Positive |
18 |
7 |
1 |
|
Negative |
2 |
0 |
8 |
|
Equivocal |
4 |
2 |
0 |
Note that the InBios DV IgG EIA shows significant cross-reactivity with antibodies to West Nile virus and St. Louis encephalitis virus.
The presence of nonstructural protein 1 (NS1) antigen overlaps with the DV viremic phase for the first 4 to 5 days following infection and therefore, the performance characteristics of the InBios DV NS1 EIA were compared to the Focus Diagnostics DV real-time PCR (RT-PCR), which detects RNA from all 4 DV serotypes. Seventy-seven serum samples previously evaluated by the Focus Diagnostics RT-PCR assay were also tested by the InBios DV NS1 EIA and the results are compared in Table 4 below. Discordant samples were also tested by the Platelia NS1 Ag EIA (BioRad Laboratories, Marnes-la-Coquette, France).
Table 4. Comparison of the InBios NS1 EIA to RT-PCR for DV detection
|
InBios DV NS1 EIA |
Focus Diagnostics DV RT-PCR |
|
|
|
Positive |
Negative |
|
Positive |
24 |
7(b) |
|
Negative |
1(a) |
43 |
|
Equivocal |
0 |
2(c) |
a. This sample was negative by the Platelia NS1 EIA
b. Five samples were also positive by the Platelia NS1 EIA
c. One sample was negative and 1 sample was indeterminate by the Platelia NS1 EIA
Sensitivity: 96% (24/25); 95% CI: 79.1%-100%
Specificity: 82.7% (43/52); 95% CI: 70.1%-90.9%
Overall Agreement: 87.1% (67/77); 95% CI: 77.6%-93%
Day(s) Performed
Tuesday
Report Available
Same day/1 to 7 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86790 x 3
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| DENVP | Dengue Virus Ab/Ag Panel, S | 104595-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| INT69 | Dengue Interpretation | 69048-7 |
| DENG | Dengue Virus Ab, IgG, S | 29661-6 |
| DENM | Dengue Virus Ab, IgM, S | 29663-2 |
| DENS1 | Dengue NS1 Ag, S | 75377-2 |
Specimen Retention Time
14 daysForms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.
Testing Algorithm
For more information see Mosquito-borne Disease Laboratory Testing.