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HelpTest Code DENGS Dengue Virus, Molecular Detection, PCR, Serum
Ordering Guidance
The presence of dengue virus nucleic acid in serum overlaps with the presence of dengue virus nonstructural protein 1 (NS1) antigen (DNSAG / Dengue Virus NS1 Antigen, Serum). Patients with a history of symptoms for more than 1 week may be negative by molecular tests (ie, real-time polymerase chain reaction) and may require serologic testing (DENVP / Dengue Virus Antibody/Antigen Panel, Serum) to confirm the diagnosis of dengue virus infection.
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container: Sterile container
Specimen Volume: 0.5 mL
Collection Instructions:
1. Within 2 hours of collection, centrifuge and aliquot the serum into a sterile container.
2. Serum specimens not aliquoted from the serum gel collection tube into a sterile container will be rejected.
Useful For
Aiding in the diagnosis of acute infection caused by dengue virus
Special Instructions
Reporting Name
Dengue Virus, PCR, SerumSpecimen Type
SerumSpecimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 7 days | |
| Frozen | 7 days |
Reject Due To
| Gross hemolysis | Reject |
| Heat-inactivated specimen | Reject |
Reference Values
Negative
Reference values apply to all ages.
Supportive Data
Assay Inclusivity:
The Altona RealStar Dengue virus reverse transcription polymerase chain reaction (RT-PCR) assay was tested using control strains of each of the 4 dengue serotypes and was able to detect serotypes 1, 2, 3 and 4.
Accuracy:
A commercial panel (SeraCare) of known positive samples for dengue virus serotypes 1, 2, 3, and 4 was tested. Each member of the panel was tested in triplicate, and all replicates were positive by the Altona RealStar Dengue assay.
Thirty analyte-negative serum samples were spiked (1:10 dilution) with plasma samples collected in South America during an outbreak of dengue virus and determined to be positive for the virus. Of the 30 spiked serum samples, 29 (97%) were positive by the Altona RealStar Dengue RT-PCR assay.
Limit of Detection:
The limit of detection in serum was determined to be the following:
Dengue serotype 1:14 genomic targets/mcL (7000 genomic targets/mL)
Dengue serotype 2: 2 genomic targets/mcL (1000 genomic targets/mL)
Dengue serotype 3: 1.6 genomic targets/mcL (800 genomic targets/mL)
Dengue serotype 4: 13 genomic targets/mcL (6500 genomic targets/mL)
Reference Range (Analytical Specificity):
A total of 20 serum samples collected from normal donors were analyzed by the Altona RealStar Dengue RT-PCR assay, and all 20 were negative.
A cross-reactivity panel of bacteria (n=12), viruses (n=15), and parasites (n=2) was tested, and all were negative by the Altona Dengue RT-PCR assay.
Day(s) Performed
Tuesday, Thursday
Report Available
Same day/1 to 5 daysSpecimen Retention Time
7 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| DENGS | Dengue Virus, PCR, Serum | 7855-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 606372 | Dengue Virus, PCR, Serum | 7855-0 |
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244)with the specimen.