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HelpTest Code DENGM Dengue Virus Antibody, IgG and IgM, Serum
Useful For
Aiding in the diagnosis of dengue virus infection
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| DENG | Dengue Virus Ab, IgG, S | No | Yes |
| DENM | Dengue Virus Ab, IgM, S | No | Yes |
| DNABI | Dengue Ab Interpretation | No | Yes |
Reporting Name
Dengue Virus Ab, IgG and IgM, SSpecimen Type
SerumSpecimen Required
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Specimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 14 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
| Heat-inactivated specimen | Reject |
Reference Values
Dengue virus antibody, IgG: Negative
Dengue virus antibody, IgM: Negative
Reference values apply to all ages.
Supportive Data
A total of 200 prospective serum samples submitted for dengue virus (DV) IgM and IgG testing by the Focus Diagnostics DV IgM and IgG enzyme immunoassays (EIA) were also tested by the InBios IgM and IgG DV assays. The results were compared and the data summarized in Tables 1 and 2.
Table 1. Comparison of the InBios and Focus (Quest) Diagnostics DV IgM EIA
|
InBios DV IgM EIA |
Focus (Quest) Diagnostics DV IgM EIA |
|
|
Positive |
Negative |
|
|
Positive |
14 |
0 |
|
Negative |
1 |
184 |
|
Equivocal |
1 |
0 |
Sensitivity: 87.5% (14/16); 95% CI: 62.7%-97.7%
Specificity: 100% (184/184); 95% CI: 97. 5%-100%
Agreement: 99% (198/200); 95% CI: 96.1%-99.9%
Table 2. Comparison of the InBios and Focus (Quest) Diagnostics DV IgG EIA
|
InBios DV IgG EIA |
Focus (Quest) Diagnostics DV IgG EIA |
|
|
|
Positive |
Negative |
|
Positive |
34 |
0 |
|
Negative |
0 |
164 |
|
Equivocal |
2 |
0 |
Sensitivity: 94.4% (34/36); 95% CI: 80.9%-99.4%
Specificity: 100% (164/164); 95% CI: 97.2%-100%
Agreement: 99% (198/200); 95% CI: 96.1%-99.9%
An additional 42 serum samples positive for IgG-class antibodies to West Nile virus (n=24), St. Louis encephalitis virus (n=9), and California (LaCrosse) virus (n=9) were also tested by the InBios DV IgG EIA and the data are summarized below in Table 3.
Table 3. Cross-reactivity of the InBios DV IgG EIA with antibodies to West Nile virus, St. Louis encephalitis virus, and California (LaCrosse) virus
|
InBios DV IgG EIA |
West Nile virus IgG positive |
St. Louis encephalitis virus positive |
California (LaCrosse) virus positive |
|
Positive |
18 |
7 |
1 |
|
Negative |
2 |
0 |
8 |
|
Equivocal |
4 |
2 |
0 |
Note that the InBios DV IgG EIA shows significant cross-reactivity with antibodies to West Nile virus and St. Louis encephalitis virus.
Day(s) Performed
Tuesday
Report Available
Same day/1 to 7 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
IgM-86790
IgG-86790
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| DENGM | Dengue Virus Ab, IgG and IgM, S | 87546-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| DENG | Dengue Virus Ab, IgG, S | 29661-6 |
| DENM | Dengue Virus Ab, IgM, S | 29663-2 |
| DNABI | Dengue Ab Interpretation | 69048-7 |
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.