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HelpTest Code CIMT Coccidioides immitis/posadasii, Molecular Detection, PCR, Paraffin, Tissue
Useful For
Rapid detection of Coccidioides DNA
Aiding in the diagnosis of coccidioidomycosis using paraffin-embedded tissue specimens
Disease States
- Coccidioidomycosis
Reporting Name
Coccidioides PCR, FFPESpecimen Type
Tissue, ParaffinNecessary Information
Specimen source is required.
Specimen Required
Preferred Paraffin-embedded tissue block:
Supplies: Tissue Block Container (T553)
Specimen Type: Formalin-fixed, paraffin-embedded tissue block (FFPE)
Sources: Body tissue
Container/Tube: Tissue block
Collection Instructions: Submit a formalin-fixed, paraffin-embedded tissue block to be cut and returned.
Acceptable Paraffin-embedded tissue block:
Specimen Type: Formalin-fixed, paraffin-embedded tissue block (FFPE)
Sources: Body tissue
Container/Tube: Sterile container for each individual cut section (scroll).
Collection Instructions: Perform microtomy and prepare five separate 10-micron sections. Each section (scroll) must be placed in a separate sterile container for submission.
Specimen Minimum Volume
See Specimen Required.
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Tissue, Paraffin | Ambient (preferred) | ||
| Refrigerated | |||
Reject Due To
| Any non-FFPE tissue blocks FFPE bone marrow FFPE slides FFPE body fluids | Reject |
Reference Values
Not applicable
Supportive Data
The sensitivity and specificity of the assay testing 148 formalin-fixed, paraffin-embedded tissue specimens was 73.4% and 100% respectively.
The limit of detection of the assay was determined to be between 1 and 10 copies of target/microliter (<50 copies of target/reaction). The analytic specificity of the assay was determined by performing a Basic Local Alignment Search Tool (BLAST) search of the primer and probe sequences on the National Center for Biotechnology Information website (http://www.ncbi.nml.nig.gov). In addition, an extensive panel of nucleic acid extracted from 114 potentially cross-reacting organisms including fungi, bacteria, mycobacteria, viruses, and human DNA was tested. The assay did not demonstrate cross-reactivity with any of the organisms included in the specificity panel.
Day(s) Performed
Monday through Sunday
Report Available
5 to 7 daysSpecimen Retention Time
7 days; after which time the block will be returned to the clientPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CIMT | Coccidioides PCR, FFPE | 95913-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| CIRR | Coccidioides PCR, FFPE, Result | 95913-0 |
| SRCCI | Coccidioides PCR, FFPE, Source | 31208-2 |