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Test Code CHIKV Chikungunya IgM and IgG, Antibody, Serum

Useful For

Aiding in the diagnosis of recent infection with Chikungunya virus in patients with recent travel to endemic areas and a compatible clinical syndrome

Profile Information

Test ID Reporting Name Available Separately Always Performed
CHIKM Chikungunya IgM, Ab, S No Yes
CHIKG Chikungunya IgG, Ab, S No Yes
CHIKI Chikungunya Interpretation No Yes

Reporting Name

Chikungunya IgM and IgG, Ab, S

Specimen Type

Serum


Ordering Guidance


Testing a patient in a convalescent period is recommended because specimens collected too early following infection may be negative for antibodies to Chikungunya virus.



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 30 days
  Frozen  30 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Reference Values

IgM: Negative

IgG: Negative

Reference values apply to all ages.

Supportive Data

Accuracy:

IgM Antibodies to Chikungunya Virus:

Originally 87 serum samples tested by the Focus Diagnostics Inc. anti-Chikungunya virus IgM immunofluorescence assay (IFA) were also evaluated by the EuroImmun anti-Chikungunya virus IgM enzyme-linked immunofluorescence assay (ELISA) and the results are indicated in Table 1.

 

Table 1. Comparison of the EuroImmun ChikV IgM ELISA and the Focus Diagnostics ChikV IgM IFA

 

 

Focus Diagnostics ChikV IgM IFA

Positive

Negative

EuroImmun ChikV IgM EIA

Positive

43

0

Negative

3

41

Borderline

0

0

Positive Agreement: 93.5 (43/46); 95% CI: 81.9%-98.4%

Negative Agreement: 100% (41/41); 95% CI: 89.8%-100%

Overall Agreement: 96.6% (84/87); 95% CI: 89.9%-99.2%

 

IgG Antibodies to Chikungunya Virus:

Originally 101 serum samples tested by the Focus Diagnostics Inc. anti-Chikungunya virus IgG IFA were also evaluated by the EuroImmun anti-Chikungunya virus IgG ELISA and the results are indicated in Table 2.

 

Table 2. Comparison of the EuroImmun ChikV IgG ELISA and the Focus Diagnostics ChikV IgG IFA

 

 

Focus Diagnostics ChikV IgG IFA

Positive

Negative

EuroImmun ChikV IgG EIA

Positive

39

2

Negative

7*

50

Borderline

0

3

 

*All 7 samples were positive by both the Focus and EuroImmun IgM assays. Also, 4 of 7 samples had low titers (≤1:20) by the IFA assay.

Positive Agreement: 84.8 (39/46); 95% CI: 71.5%-92.7%

Negative Agreement: 90.9% (50/55); 95% CI: 80.0%-96.5%

Overall Agreement: 88.1% (89/101); 95% CI: 80.2%-93.2%

 

Reference Range:

Of serum samples collected from normal donors, 74/75 (98.7%) and 90/90 (100%) were negative by the EuroImmun anti-Chikungunya virus IgG and IgM assays, respectively.

 

Analytical Specificity:

1. Sixty serum samples previously characterized as positive for IgG-class antibodies to West Nile virus (n=29), Dengue virus (n=15), St. Louis encephalitis virus (n=8), California encephalitis virus (n=6), and Western equine encephalitis virus (n=2) were analyzed by the EuroImmun anti-Chikungunya virus IgG assay. One sample, positive for IgG antibodies to Dengue virus was also positive by the Chikungunya IgG assay, giving an overall specificity of 98.3% (59/60).

 

2. Thirty-three serum samples previously characterized as positive for IgM-class antibodies to West Nile virus (n=8), Dengue virus (n=11), St. Louis encephalitis virus (n=6), California encephalitis virus (n=6), and Western equine encephalitis virus (n=2), were analyzed by the EuroImmun anti-Chikungunya virus IgM assay. Two samples, positive for IgM antibodies to Dengue virus were also positive by the Chikungunya IgM assay, giving an overall specificity of 93.9% (31/33).

 

Note: Dengue and Chikungunya virus cocirculate in endemic areas and are transmitted by the same mosquito genera, so the 3 specimens with antibodies to both viruses may indicate coinfection or past exposure to both viruses.

Day(s) Performed

Bimonthly on the second and fourth Wednesday; fifth Wednesday when applicable

Report Available

Same day/1 to 14 days

Specimen Retention Time

14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86790 x2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CHIKV Chikungunya IgM and IgG, Ab, S 93976-9

 

Result ID Test Result Name Result LOINC Value
CHIKI Chikungunya Interpretation 69048-7
CHIKG Chikungunya IgG, Ab, S 88630-9
CHIKM Chikungunya IgM, Ab, S 88629-1

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.

Testing Algorithm

For more information see Mosquito-borne Disease Laboratory Testing.