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Test Code CEE52 CD52 Cell Expression Evaluation, Varies


Ordering Guidance


This test should not be used as a shortened diagnostic panel. For a complete diagnostic B-cell, T-cell, or acute immunophenotyping panel, order LCMS / Leukemia/Lymphoma Immunophenotyping, Flow Cytometry, Varies.

 

This test evaluates CD52 expression only. For CD20 expression, order CEE20 / CD20 Cell Expression Evaluation, Varies. For CD49d expression, order CEE49 / CD49d Cell Expression Evaluation, Varies.



Shipping Instructions


Specimen must arrive within 4 days of collection.



Necessary Information


The following information is required:

1. The therapeutic monoclonal antibody being used or considered

2. The pertinent hematologic diseases that have been diagnosed or considered

3. Diagnostic immunophenotype of the neoplastic cells (when available, a pathology and/or flow cytometry report should be included).

4. Specimen source

5. Date and time of collection



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Whole Blood

Container/Tube:

Preferred: Yellow top (ACD solution A or B)

Acceptable: Lavender top (EDTA) or Green top (sodium heparin) 

Specimen Volume: 10 mL

Collection Instructions:

1. Send specimen in original tube. Do not aliquot.

2. Label specimen as blood.

Specimen Stability Information: Ambient/Refrigerated ≤4 days

 

Specimen Type: Bone marrow

Container/Tube:

Preferred: Yellow top (ACD solution A or B)

Acceptable: Lavender top (EDTA) or Green top (sodium heparin) Specimen Volume: 1-5 mL

Collection Instructions:

1. Submission of bilateral specimens is not required.

2. Label specimen as bone marrow.

3. Send bone marrow specimen in original tube. Do not aliquot.

Specimen Stability Information: Ambient/Refrigerated ≤4 days


Forms

If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.

Useful For

Detecting cell-surface antigens on malignant cells that are potential therapeutic antibody targets, specifically CD52

 

Determining the eligibility of patients for monoclonal antibody therapies

 

Monitoring response to the therapeutic antibody

Testing Algorithm

A complete diagnostic B-cell, T-cell, or acute immunophenotyping panel is not performed. In some cases, a limited morphologic evaluation will be performed.

Reporting Name

CD52 Cell Expression Evaluation, V

Specimen Type

Varies

Specimen Minimum Volume

Blood: 3 mL
Bone Marrow Aspirate: 1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Varies 4 days

Reject Due To

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Reference Values

Normal individuals have B lymphocytes, T lymphocytes, or myeloid cells that express the corresponding cell-surface antigens in question.

Day(s) Performed

Monday through Saturday

Report Available

1 to 4 days

Specimen Retention Time

14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

88184-Flow cytometry; first cell surface, cytoplasmic or nuclear marker

88185 x 3-Flow cytometry; additional cell surface, cytoplasmic or nuclear marker

88187-Flow Cytometry Interpretation, 2 to 8 Markers

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CEE52 CD52 Cell Expression Evaluation, V 100991-9

 

Result ID Test Result Name Result LOINC Value
CK166 CEE52 Result No LOINC Needed
CK167 Final Diagnosis 22637-3