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HelpTest Code CBSRP Coxiella burnetii (Q Fever), Molecular Detection, PCR, Serum
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
Coxiella burnetii (Q fever) PCR, SSpecimen Type
SerumSpecimen Required
The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Coxiella burnetii DNA is unlikely.
Collection Container/Tube:
Preferred: Red top
Acceptable: Serum gel
Submission Container/Tube: Sterile vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a sterile vial within 2 hours of collection.
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Frozen (preferred) | 7 days | |
| Refrigerated | 7 days |
Specimen Minimum Volume
0.5 mL
Day(s) Performed
Monday through Friday
Specimen Retention Time
7 daysReport Available
2 to 7 daysReference Values
Not applicable
Useful For
Aiding in the diagnosis of Coxiella burnetii infection (ie, Q fever) using serum specimens
CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CBSRP | Coxiella burnetii (Q fever) PCR, S | 90443-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 35189 | Specimen Source | 31208-2 |
| 35190 | Coxiella burnetii PCR | 90443-3 |
Supportive Data
This assay was clinically validated in a blinded manner using 52 archived, formalin-fixed, paraffin-embedded heart valve specimens from patients with endocarditis. A single sample within this set was determined to contain polymerase chain reaction (PCR) inhibitors and omitted from the final analysis set. Compared with existing diagnostic data, PCR had a sensitivity of 100% (8/8) and specificity of 100% (43/43). All samples were assayed with a second PCR assay targeting the IS1111 element.(1) Complete concordance was noted between the 2 assays (P >0.999). The limit of detection of the assay is 21.6 targets/mcL for serum.
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.