Sign in →

Test Code CBBRP Coxiella burnetii (Q fever), Molecular Detection, PCR, Blood

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Reporting Name

Coxiella burnetii (Q fever) PCR, B

Specimen Type

Whole Blood EDTA


Ordering Guidance


 



Specimen Required


The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Coxiella burnetii DNA is unlikely.

 

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Royal blue top (EDTA), pink top (EDTA), or sterile vial containing EDTA-derived aliquot

Specimen Volume: 1 mL

Collection Instructions: Send whole blood specimen in original tube (preferred).


Reject Due To

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Refrigerated (preferred) 7 days
  Frozen  7 days

Specimen Minimum Volume

0.5 mL

Day(s) Performed

Monday through Friday

Specimen Retention Time

1 week

Report Available

Same day/1 to 4 days

Reference Values

Not applicable

Useful For

Aiding in the diagnosis of Coxiella burnetii infection (eg, Q fever)

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CBBRP Coxiella burnetii (Q fever) PCR, B 90443-3

 

Result ID Test Result Name Result LOINC Value
35191 Specimen Source 31208-2
35192 Coxiella burnetii PCR 90443-3

Supportive Data

This assay was clinically validated in a blinded manner using 52 archived, formalin-fixed, paraffin-embedded heart valve specimens from patients with endocarditis. A single sample within this set determined to contain polymerase chain reaction (PCR) inhibitors was omitted from the final analysis set. Compared with existing diagnostic data, PCR had a sensitivity of 100% (8/8) and specificity of 100% (43/43). All samples were assayed with a second PCR assay targeting the IS1111 element.(1) Complete concordance was noted between the 2 assays (P >0.999). The limit of detection of the assay is 2.16 targets/mcL for EDTA whole blood.

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.