Ordering Guidance

If testing to evaluate C5b-9 in the context of atypical hemolytic uremic syndrome is desired, see AHUSD / Atypical Hemolytic Uremic Syndrome Complement Panel, Serum and Plasma.

Specimen Required

Patient Preparation:

1. Fasting preferred but not required.

2.  Do not collect specimens for at least 48 hours following plasma exchange.

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Lavender top (K2 EDTA)

Acceptable: Lavender top (K3 EDTA), light-blue top (sodium citrate)

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions:

1. Immediately after specimen collection, place the tube on wet ice.

2. Centrifuge between 1000 and 2000 x g for 10 minutes at 4° C and aliquot plasma into a plastic vial.

3. Within 30 minutes of centrifugation, freeze specimen. Sample must be placed on dry ice if not frozen immediately.

NOTE: If a refrigerated centrifuge is not available, it is acceptable to use a room temperature centrifuge, provided the sample is kept on ice before centrifugation, and immediately afterward, the plasma is aliquoted and frozen.