Sign in →

Test Code C5B9 SC5b-9 Level Terminal Complement Complex, Plasma


Ordering Guidance


If testing to evaluate C5b-9 in the context of atypical hemolytic uremic syndrome is desired, see AHUSD / Atypical Hemolytic Uremic Syndrome Complement Panel, Serum and Plasma.



Specimen Required


Patient Preparation:

1. Fasting: 8 hours, preferred but not required

2. Do not collect specimens for at least 48 hours following plasma exchange.

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Lavender top (K2 EDTA)

Acceptable: Lavender top (K3 EDTA), light-blue top (sodium citrate)

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL Plasma

Collection Instructions:

1. Immediately after specimen collection, place the tube on wet ice.

2. Centrifuge between 1000 and 2000 x g for 10 minutes at 4° C.

3. Aliquot plasma into a plastic vial.

4. Within 30 minutes of centrifugation, freeze specimen. Specimen must be placed on dry ice if not frozen immediately.

Note: If a refrigerated centrifuge is not available, it is acceptable to use a room temperature centrifuge, provided the sample is kept on ice before centrifugation, and immediately afterward, the plasma is aliquoted and frozen.


Useful For

Detecting increased complement activation

Reporting Name

SC5b-9 Complement, P

Specimen Type

Plasma EDTA

Specimen Minimum Volume

Plasma: 0.75 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma EDTA Frozen 14 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Reference Values

≤250 ng/mL

Method Description

Standards, controls, and test specimens are added to microassay wells coated with mouse monoclonal antibody that binds specifically to the C9 ring of SC5b-9 activation complex. A wash cycle removes unbound material following incubation. Horseradish peroxidase (HRP)-conjugated goat antibodies to antigens of SC5b-9 are added to each well and will bind to SC5b-9 that was captured on the surface of the microassay wells. A wash cycle removes unbound, excess conjugate. A chromogenic enzyme substrate is added to each microassay well. The bound HRP-conjugate reacts with the substrate forming a blue color. After incubation, the enzyme reaction is stopped chemically, and the color intensity is measured spectrophotometrically at 450 nm. The color intensity of the reaction mixture is proportional to the concentration of SC5b-9 complex present.(Package insert: MicroVue SC5b-9 Plus EIA Kit. Quidel Corporation; PIA020004EN00, 06/2022)

Day(s) Performed

Varies

Report Available

3 to 5 days

Specimen Retention Time

14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86160

LOINC Code Information

Test ID Test Order Name Order LOINC Value
C5B9 SC5b-9 Complement, P 93244-2

 

Result ID Test Result Name Result LOINC Value
616921 SC5b-9 Complement, P 93244-2